Practical

Paper Code: 
BOT 503
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00

Suggested exercises:

  • Demonstration of phemomenon of plasmolysis using Rheo discolor leaves.
  • Demonstration of phemomenon of osmosis by potato osmoscope.
  • Demonstration of phemomenon of imbibition.
  • Demonstration of phemomenon of root pressure.
  • Demonstration of phemomenon of unequal transpiration in dorsiventral leaves using cobalt chloride paper.
  • Measurement of the rate of transpiration using potometers.
  • Anatomical study of root nodule depicting symbiotic relationship between nitrogen fixing bacteria and roots of leguminuous plants.
  • To demonstrate that light, CO2 and chlorophyll are necessary for photosynthesis.
  • To demonstrate that O2 is evolved during photosynthesis.
  • To determine the value of RQ of different respiratory substrates by Ganong’s respiroscope.
  • To demonstrate that CO2 is given out during respiration
  • Measurement of growth by arc auxanometer.
  • Phytochemical tests for the following- glucose, starch, proteins, fats, tannins, ascorbic acid and anthocyanins.
  • Demonstration of respiratory enzymes- peroxidase, catalase and dehydrogenase in plant tissue samples and measurement of their activities.
     
References: 
  1. Ramakrishnan, S; Prasannan, KG and Rajan, R. Textbook of Medical Biochemistry (2nd edition). Orient Longman.
  2. Methods in Plant Biochemistry and Molecular Biology. 1997. Dashek, WV (ed.). CRC Press.
  3. Wilson and Walker .Practical Biochemistry: Principles and Techniques. Cambridge
  4. University Press.U.K.
     
Academic Year: