Practical
Suggested exercises:
- Demonstration of phemomenon of plasmolysis using Rheo discolor leaves.
- Demonstration of phemomenon of osmosis by potato osmoscope.
- Demonstration of phemomenon of imbibition.
- Demonstration of phemomenon of root pressure.
- Demonstration of phemomenon of unequal transpiration in dorsiventral leaves using cobalt chloride paper.
- Measurement of the rate of transpiration using potometers.
- Anatomical study of root nodule depicting symbiotic relationship between nitrogen fixing bacteria and roots of leguminuous plants.
- To demonstrate that light, CO2 and chlorophyll are necessary for photosynthesis.
- To demonstrate that O2 is evolved during photosynthesis.
- To determine the value of RQ of different respiratory substrates by Ganong’s respiroscope.
- To demonstrate that CO2 is given out during respiration
- Measurement of growth by arc auxanometer.
- Phytochemical tests for the following- glucose, starch, proteins, fats, tannins, ascorbic acid and anthocyanins.
- Demonstration of respiratory enzymes- peroxidase, catalase and dehydrogenase in plant tissue samples and measurement of their activities.
References:
- Ramakrishnan, S; Prasannan, KG and Rajan, R. Textbook of Medical Biochemistry (2nd edition). Orient Longman.
- Methods in Plant Biochemistry and Molecular Biology. 1997. Dashek, WV (ed.). CRC Press.
- Wilson and Walker .Practical Biochemistry: Principles and Techniques. Cambridge
- University Press.U.K.