Tools and Techniques of Plant Science.

Paper Code: 
BOT323
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course Objectives:

This course will enable the students to –

  1. The course aims at studying not only morphology, structure and development of flowering plants but at their practical and experimental aspects as they are most sought after areas of modern research.
  2. To enhance the fundamental knowledge about living cells and their systematic up gradation in to the tissue level.
  3. To study the differentiation features of internal structure of the plant body.
  1. To obtain a thorough knowledge about the development cycle in higher plants

Course Outcomes (COs):

     Course

Course Outcome

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BOT 323

 

Tools and Techniques of Plant Science

CO66:  Develop conceptual understanding of cell wall degradation enzymes and cell fractionation.

CO67:  Classify different types of chromatography techniques.

CO68:  Explain the principles of light microscopy, compound microscopy, fluorescence microscopy and confocal microscopy

CO69:  Apply suitable strategies for data collections and disseminating research findings.

CO70:  Learn the methods of Biostatistics and its application in Biology.

CO71:  Gain theoretical as well as practical knowledge to be able to find employment in research fields associated with biosciences

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question preparation

 

 

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

 

 

12.00
Unit I: 
Biostatistics:

Biostatistics: Scope of Biostastistics, exploring and presenting data: scales of measurement (nominal and ordinal), tables and graphs for nominal, ordinal and numerical data, summarizing data: measure of central tendency and dispersion

 

12.00
Unit II: 
Microscopy:

Microscopy: Magnifiers and microscopes, simple and compound microscopes, bright field and dark field microscopy, confocal and deconvolution  microscopy, microscopic measurements, phase contrast, fluorescence microscopy, electron microscopy – SEM, TEM. 

 

12.00
Unit III: 
Histology of Tissues

Histology of Tissues: Selection of material, chemical fixation – types of fixatives, mechanism of fixation, tissue dehydration – general protocol, rapid dehydration and dehydration using a graded solvent series of ethanol or acetone, infiltrating and embedding tissues, sectioning, mounting and staining, alternate methods of microtomy: cryotome  and vibratome.

12.00
Unit IV: 
Basic Techniques

Basic Techniques: Agarose gel electrophoresis, SDS-PAGE, isolation and purification of genomic DNA, elution from gel, Blotting (Southern, Northern and Western), HPLC, Autoradiography.                                                              

 

12.00
Unit V: 
Microbiological and Immunotechniques

Microbiological and Immunotechniques: Sterilization and disinfection: Preparation of media, sterilization, culture and transfer instruments, cultivation chambers, culture transfer techniques, pure culture techniques: streak plate, pour plate and spread plate method, media preparation-solid and liquid media, simple and complex media, nutrient agar slabs, slants and plates, staining techniques, simple and differential staining, enumeration using haemocytometer, bio chemical characterization – TSI test, IMViC test, ELISA, Radio Immunoassay (RIA), Ouchterlony latex agglutination test, monoclonal and polyclonal antibody production

 

Essential Readings: 

·                     A Biology Guide to Principles and Techniques of Practical Biochemistry. Wilson,   & Goulding, KH. ELBS edition.

·                     Introduction to Instrumental Analysis. Robert Brown. Mc Graw Hill Internatiuonal Edition.

·                     Introduction to Practical Molecular Biology. Dabre, PG. John Wiley & Sons Ltd.

·                     Kuby Immunology (fourth edition). Golds, RA. Thomas J. Kintz, Barbara, A. Osborne, Freeman & Co., New York.

·                     Microbiological Applications: A Laboratory Manual in General Microbiology. Benson, HJ. WCG; WnC Brown Publishers.

·                     Microbiology, a Laboratory Manual. Cappuccino, JG and Sherman, N. Addison Wesley.

·                     Molecular and Cellular Methods in Biology & Medicine. 1995. Win, K, Kim & Cseke. CRC Press, Florida 

Molecular Cloning: A Laboratory Manual. Sambrook, J Fritsch, EF & Maniatis, T. Cold Spring Harbor Laboratory Press, New York

Academic Year: