Course Objectives:
This course will enable the students to –
Course Outcomes (COs):
Course |
Course Outcome |
Learning and teaching strategies |
Assessment Strategies |
|
Paper Code |
Paper Title |
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BOT 323
|
Tools and Techniques of Plant Science |
CO66: Develop conceptual understanding of cell wall degradation enzymes and cell fractionation. CO67: Classify different types of chromatography techniques. CO68: Explain the principles of light microscopy, compound microscopy, fluorescence microscopy and confocal microscopy CO69: Apply suitable strategies for data collections and disseminating research findings. CO70: Learn the methods of Biostatistics and its application in Biology. CO71: Gain theoretical as well as practical knowledge to be able to find employment in research fields associated with biosciences |
Class lectures Seminars Tutorials Group discussions and Workshops Question preparation
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Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects |
Microscopy: Magnifiers and microscopes, simple and compound microscopes, bright field and dark field microscopy, confocal and deconvolution microscopy, microscopic measurements, phase contrast, fluorescence microscopy, electron microscopy – SEM, TEM.
Histology of Tissues: Selection of material, chemical fixation – types of fixatives, mechanism of fixation, tissue dehydration – general protocol, rapid dehydration and dehydration using a graded solvent series of ethanol or acetone, infiltrating and embedding tissues, sectioning, mounting and staining, alternate methods of microtomy: cryotome and vibratome.
· A Biology Guide to Principles and Techniques of Practical Biochemistry. Wilson, & Goulding, KH. ELBS edition.
· Introduction to Instrumental Analysis. Robert Brown. Mc Graw Hill Internatiuonal Edition.
· Introduction to Practical Molecular Biology. Dabre, PG. John Wiley & Sons Ltd.
· Kuby Immunology (fourth edition). Golds, RA. Thomas J. Kintz, Barbara, A. Osborne, Freeman & Co., New York.
· Microbiological Applications: A Laboratory Manual in General Microbiology. Benson, HJ. WCG; WnC Brown Publishers.
· Microbiology, a Laboratory Manual. Cappuccino, JG and Sherman, N. Addison Wesley.
· Molecular and Cellular Methods in Biology & Medicine. 1995. Win, K, Kim & Cseke. CRC Press, Florida
Molecular Cloning: A Laboratory Manual. Sambrook, J Fritsch, EF & Maniatis, T. Cold Spring Harbor Laboratory Press, New York