Practicals based on BOT 321, BOT 322, BOT 323 & BOT 324

Paper Code: 
BOT325
Credits: 
8
Contact Hours: 
240.00
Max. Marks: 
100.00
Objective: 

Course Objective:

This course will enable the students to –

  1. To demonstrate procedural knowledge that creates different types of professionals in the field of Botany like in research and development, teaching government and public services. 

     Course

Course Outcome

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BOT325

 

Practical

CO80:  Field study of a floristic rich area to observe and collect the plants from their natural habitats.

CO81:  Exploration of gross anatomical details of cells, tissues and various other organs of plants.

CO82:  Compare, contrast, draw, and describe the taxonomic and evolutionary variation in xylem and phloem components.

CO83:  Outline and describe current understanding of the components of shoot, root, and floral development.

CO84:  Conceptual learning from permanent preparation of slides like anther, male gametophyte, different types of ovules.

CO85:  Basic knowledge about the plant specimen from members of locally available dicotyledonous and monocotyledonous families

CO86:  Learn how to describe the specimens with preparation of keys up to generic level of locally available plants.

CO87:  Learn how to prepare media for tissue culture, isolation of protoplast and organogenesis.

 

Substantial laboratory-based practical component and experiments

Technology-enabled learning

Field-based learning

Project-based learning

 

Practical assignments and laboratory reports

Observation of practical skills

Individual and group project reports

 

Viva voce interviews are majorly adopted assessment methods

 

 
 

 

Unit I: 
Practicals based on BOT 321
  • Field visit to identify various life forms & species constituting the local flora.
  • Study of representative / available members of the families mentioned in the syllabus.
  • Collection and drying of specimen to prepare herbaria.
  • To study problems in numerical taxonomy.
  •  To retrieve literature using GIS

 

 

Unit II: 
Practicals based on BOT 322
  • L-S root and shoot tip to study cytohistological zonation.
  • Anatomy of primary and secondary growth in monocot and dicot root & stem.
  • Study of growth rings in wood. Microscopic study of wood in T.S, T.L.S and R.L.S.
  • Anatomy of monocot and dicot leaf.
  • Study of various types of stomata in leaf epidermal peels.
  • Study of different pollination mechanism in flowers.
  • Test of self incompatibility using field pollinations.
  • Emasculation and bagging technique to prevent self fertilization.
  • Structure of anther and pollen grains with special reference to microsporangium wall features.
  • Pollen viability using in vitro pollen germination.
  • Structure of different types of ovules and embryo sacs.
  • Study of endosperm and embryo.
  • To study germination of seeds, both dormant and non-dormant
  • To study polyembryony in orange seeds.

 

 

Unit III: 
Practicals based on BOT 323
  • Demonstration of simple and compound microscope
  • Demonstration of methods of histology of tissue- dehydration, sectioning, mounting
  • Demonstration of following immunotechniques- ELISA, RIA, Ouchterlony.
  • Demonstration of SDS-PAGE, HPLC
  • Demonstration of following Microbiological techniques- Sterilization, Pure culture techniques, Haemocytometer, Staining Techniques.
  • Demonstration of gel electrophoresis.

 

Unit IV: 
Practicals based on BOT 324
  • Media preparation, sterilization and inoculation of given material.
  • Growth characteristics of E.coli using plating and turbidimetric methods.
  • Isolation of plasmid from E.coli by alkaline lyses method and its quantization spectrophotometrically.
  • Restriction digestion of the plasmid and estimation of the size of various DNA fragments.
  • Cloning of a DNA fragment in plasmid vector, transformation of the given bacterial population and selection recombinants.
  • Organogenesis and somatic embryogenesis using appropriate explants and preparation of artificial seeds.
  • Isolation of protoplast from various plant tissues and testing their viability.
  • Effect of physical and chemical factors protoplast yield.
  • Demonstration of protoplast fusion employing PEG.
  • Demonstration of DNA sequencing using Sanger’s dideoxy method.
  • Demonstration of micro techniques for plant cultures.
  • Co-cultivation of the plant material with Agrobacterium and study GUS activity histochemically.

 

 

 

 

Essential Readings: 

·         Bendre. 1995. A Text Book of Practical Botany. Rastogi Publications, Meerut.

·         Pandey, BP and Chadha. 1997. Botany Vol. III. Vikas Publishing House

·         Sharma, K. Manual of Microbiology: Tools and Techniques. 2005. Ane Books.

·         Henry, RJ. 1997. Practical Application of Plant Molecular Biology. Chapman & Hall, London

Philips, GC. 1995. Plant Cell, Tissue and Organ Culture: Fundamental Methods. Narosa Publishing House

Pandey, BP and Chadha. 1997. Botany Vol. III. Vikas Publishing House

Academic Year: 
2019-2020
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