Practical Exercises based on BOT 221-224

Paper Code: 
BOT 225
Credits: 
9
Contact Hours: 
60.00
Max. Marks: 
100.00

BOT 221:  Pteridophytes, Gymnosperms and Palaeobotany

 

  • Study of vegetative and reproductive structures of the following genera : Psilotum, Lycopodium, Selaginella , Isoetes , Equisetum ,Ophioglossum , Marsilea. Study of vegetative and reproductive structures of the following genera: Cycas, Ginkgo , Abies, Pinus, Picea , Taxus, Cryptomeria, Araucaria , Cephalotaxus, Cupressus, Podocarpus, Ephedra, Welwitschia, Gnetum
  • Study of the following genera  : Rhynia, Horneophyton, Lyginopteris, Medullosa, Neuropteris, Glossopteris.

 

 

BOT 222:  Environmental Science

 

  • To determine minimum number of quadrats required for reliable estimate  of biomass in grasslands.
  • To study the frequency of herbacious species in grassland and to compare the frequency distribution with Raunkair's standard frequency diagram/
  • To estimate importance value index for grassland species on the basis of relative frequency, relative density and relative biomass in protected and grazed grassland.
  • To measure the vegetation cover of grassland through point frame method.
  • To measure the above ground plant biomass in a grassland.
  • To determine Kemps constant for dicot and monocot leaves and to estimate the leaf area index of a grassland community.
  • To determine diversity indices (richness, simpson, Shannon weinner) in grazed and protected grassland.
  • To estimate bulk density and particle density of grassland and woodland soils.
  • To determine moisture content and water holding capacity of grassland and woodland soil.
  • To study the vegetation structure through profile diagram.
  • To estimate transparency, pH, and temperature of different water bodies.
  • To measure dissolved oxygen content in polluted and unpolluted water samples.
  • To estimate salinity of different water samples.
  • To determine the percent leaf area injury of different leaf samples collected around polluted sites.
  • To estimate dust holding capacity of the leaves of different plant species

 

BOT 223: Plant Physiology and Biochemistry-I

  • Demonstration of phenomenon of osmosis by using potato osmometer.
  • Demonstration of phenomenon of imbibition.
  • Demonstration of the stomatal transpiration by four leaves method.
  • To demonstrate that oxygen is evolved during photosynthesis by inverted funnel method.
  • To demonstrate that CO2 , water, light and chlorophyll are essential for photosynthesis by ‘Moll’s half leaf’ experiment.
  • To determine the chlorophyll a / chlorophyll b ratio in C3 and C4 plants.
  • Isolation of intact chloroplasts and estimation of chloroplast proteins by spot protein assay.
  • Extraction of chloroplast pigments from leaves and preparation of the absorption spectrum of chlorophylls and carotenoids.
  • To demonstrate photophosphorylation in intact chloroplasts, resolve the phosphoproteins by SDS-PAGE and perform autoradiography.
  • To determine the value of RQ of different respiratory substrates using Ganong’s respirometer.
  • Demonstration of respiratory enzymes in plant tissues.
  • Demonstration of the substrate inducibility of the enzyme nitrate reductase.
  • Measurement of growth using auxanometer.
  • To study the effect of plant growth regulators on plant growth.

 

BOT 224: Plant Physiology and Biochemistry - II

 

  • To separate the given mixture of dyes using paper chromatography.
  • To separate the given mixture of amino acids using thin - layer chromatography.
  • To separate the given mixture of pigments using thin – layer chromatography.
  • Principles of colorimetry and spectrophotometry.
  • To estimate the conc. of  protein using  Folin’s reagent.
  • To estimate the conc. of  protein using  Biuret  reagent.
  • Desalting of proteins by gel filtration chromatography , employing SephadexG-25.
  • Preparation of the standard curve of protein (BSA) and estimation of the protein content in extracts of plant material by Lowry’s or Bradford’s method.
  • Effect of time and enzyme concentration on the rate of reaction of enzyme (e.g. acid phosphatase ).
  • Effect of substrate concentration on activity of any enzyme and determination of its Km value.
  • Microchemical tests for the following – Cellulose, Cutin, Suberin, Lignin, Latex, Glucose, Sucrose, Proteins, Fats, Starch.
  • Phytochemical tests for – Alkaloids, Terpenes, Tannins and Anthocyanins

 

 

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