Coming soon
Practical
This course will enable the students to –
- To demonstrate technical knowledge and develop skill set that creates different types of professionals in the field of Botany like in research and development, teaching government and public services.
Course Outcomes (COs):
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Course Outcome |
Learning and teaching strategies |
Assessment Strategies |
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On completion of this course, the students will be able to: CO84: Understand the basic concept of DNA isolation, SDS-PAGE and gel electrophoresis. CO85: Develop knowledge of basic cytogenetic laboratory techniques necessary to prepare tissue samples. CO86: Understand and investigate the cause and effect of chromosome abnormalities and associated human diseases. |
Substantial laboratory-based practical component and experiments Technology-enabled learning Field-based learning Project-based learning |
Practical assignments and laboratory reports Observation of practical skills Individual and group project reports
Viva voce interviews are majorly adopted assessment methods |
CONTENTS:
- Isolation of RNA from Yeast
- Restriction Digestion
- Agarose – Gel Electrophoresis
- SDS- PAGE
- Isolation of DNA from plant material
- Study of general cytological techniques
- Study of pollen viability
- To measure different types of plant cells using Stage micrometer and Ocular micrometer.
- Induction of polyploidy in plants by treating shoot apical meristem with colchicine solution.
- Demonstration of Cytogenetical techniques like physical mapping, RAPD and RFLP
