Practical

Paper Code: 
BOT 603
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to-

  1. To develop skills related to plant tissue culture and applied botany

Course Outcomes (COs):

Course

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BOT 603

 

Practical

On completion of this course, the students will be able to;

CO95:  Acquire hands on training on different techniques of plant propagation such as cutting, grafting and layering.

CO96: Explain and design various tissue culture experiments for plant propagation

CO97: Implement practical knowledge of various garden operations.

CO98: Integrate and attributing role of plants in cosmetic industry, medicine, environmental cleanup and energy sector

 

 

Substantial laboratory-based practical component and experiments

 

Technology-enabled learning

 

Field-based learning

Project-based learning

 

Practical assignments and laboratory reports

Observation of practical skills

Individual and group project reports

Viva voce interviews are majorly adopted assessment methods

 

 

  • Study of laboratory requirements and general techniques of biotechnology.
  • Principle and application of the following- Laminar Air Flow, Autoclave and Ultracentrifugation.
  • Preparation of different types of tissue culture medias and solutions of nutrients and growth regulators
  • Demonstration of inoculation technique, asceptic transfer of explant, optimizing the conditions for direct/ indirect differentiation in different explants to study totipotency, cytodifferentiation and organogenesis.
  • Germination of pollen of Catharanthus roseus in 10 % sucrose solution.
  • Demonstration of the technique of protoplast isolation and somatic embryogenesis in Daucus carota.
  • Demonstration of the technique of anther culture in Datura.
  • Demonstration of the technique of gel electrophoresis of bacterial DNA.
  • Demonstration of the phenomenon of action of Restriction Endonucleases. Field study of various plant forms- climbers, trees, shrubs, orchids, ferns and palms.
  • Experimental study of the technique of vegetative propagation-cutting, budding, grafting and air layering etc.
  • To study propagation by means of bulbs, corms, tubers, rhizome etc.
  • To study different styles of garden design.
  • Field visit to understand different patterns of landscape design.
  • Preparation of record on local biodiversity.

 

 

Essential Readings: 
  • Bendre and Kumar Vol.I Rastogi Publication
  • Home Gardening-Pratibha Trivedi
  • Applied Botany-P.C.Trivedi
  • College Botany Practical Vol. I and II - Santia S.C.
  • Gardening in India -Lancaster’s Percy

 

Academic Year: