Practical

Paper Code:

BOT 125

Credits:

Contact Hours:

60.00

Max. Marks:

100.00

Objective:

Study of general cytological techniques
Study of various stages of mitosis in Onion root tips
Study of various stages of meiosis in Onion flower bud
Calculation of mitotic index
To study B chromosomes in plants
Study of pollen viability
To measure different types of plant cells using Stage micrometer and Ocular micrometer
Induction of polyploidy in plants by treating shoot apical meristem with colchicines solution

Preparation of report on Indian contributors in the field of algal, fungal, bryophytic, gymnospermic and angiospermic paleobotany
Microscopic examination of stained cell preparation of bacteria by various staining methods
Study of role of bacteria in transformation of milk to milk products and in carbohydrate fermentation
Determination of Biological Oxygen Demand in pure water and water with algal growth to various degrees
Estimation of protein content in Spirulina, the Single Cell Protein
Study of mushroom cultivation
Study of moisture retaining capacity of peat moss
Study of economic importance of Gymnosperms
Study of different types of lichen thalli and their role in succession

Cleaning of glassware
To study different methods of sterilization and disinfection by physical agents.
Instrumentation
To study various types of culture media
To determine the growth curve in bacteria (E.coli)
To study the bacteria by Gram staining
To become acquainted with Kirby-Baur procedure
Wine production by fermentative activities of Yeast cells
Isolation of micro organisms from the mixed culture by spread plate methods
Isolation of micro organism by pour plate method
To study ecological relationship between micro organisms of soil by Wino Gradsky Column
To study the presence of faecal contamination in the given water sample
Survey of dermatological diseases caused by Fungi
Inventry preparations of antibiotics procured from Fungi
Morphological study of representative members of Fungi- Pilobolus, Chaetomium, Morchella, Melampsora, Polyporus, Dreschlera, Phoma, Peziza, Puccinia
Symptomatology, identification, causal organism of the following diseases: White rust, bunt, smut, green ear, smut and ergot of Bajra, rust and smut of wheat, paddy blast, cotton wilt, downy mildew and powdery mildew of grapes, tundu, tobacco mosaic virus, little leaf of brinjal, root knot of vegetables
Report on eminent contributors in Mycology, Plant pathology and important related institutes

Academic Year:

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