Coming soon
Practical Based on BOT 421A &BOT422A
Course Objective
This course will enable the students to –
- To demonstrate procedural knowledge that creates different types of professionals in the field of Botany like in research and development, teaching government and public services.
Course Outcomes (COs):
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Course |
Course Outcome |
Learning and teaching strategies |
Assessment Strategies |
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Paper Code |
Paper Title |
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BOT 423A
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Practical |
CO105: Learn how to prepare media for tissue culture, isolation of protoplast and organogenesis. CO106: Basic concept of protoplast isolation CO107: How to demonstrate DNA sequencing CO108: Understand basic laboratory setup, handling of explant tissue, nutrient medium and establishing the culture and incubation of cultures. CO109: Describe suitable methods for characterizing the activity, function, diversity and composition of microbial commUnities. CO110: To demonstrate the ability to identify significant biological research questions, develop research protocols and properly analyze research questions through the use of the scientific method. |
Substantial laboratory-based practical component and experiments Technology-enabled learning Field-based learning Project-based learning Internship in industry, and research establishments. Literature surveys and evaluations
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Practical assignments and laboratory reports Observation of practical skills Individual and group project reports
Viva voce interviews are majorly adopted assessment methods
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- Media preparation, sterilization and inoculation of given material.
- Growth characteristics of E.coli using plating and turbidimetric methods.
- Isolation of plasmid from E.coli by alkaline lysis method and its quantitation spectrophotometrically.
- Restriction digestion of the plasmid and estimation of the size of various DNA fragments.
- Cloning of a DNA fragment in a plasmid vector, transformation of the given bacterial population and selection recombinants.
- Organogenesis and somatic embryogenesis using appropriate explantsand preparation of artificial seeds.
- Isolation of protoplast from various plant tissues and testing their viability.
- Effect of physical and chemical factors protoplat yield.
- Demonstration of protoplast fusion employing PEG.
- Demonstration of DNA sequencing using Sanger,s dideoxy method.
- Demonstration of microtechniques for plant cultures.
- Co-cultivation of the plant material with Agrobacterium and study GUS activity histochemically.
