Plant Physiology and Biochemistry II

Paper Code: 
BOT224
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course Objectives:

This course will enable the students to –

  1. To study the various biochemical pathways in plants and to study the structure of various biomolecules.
  2. To enhance the knowledge on physiology and biochemical aspects through series of experiments
  3. To dissipate facts on the fundamental aspects of biochemical interactions specific to plants

Course Outcomes (COs):

     Course

Course Outcome

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BOT 224

 
Plant Physiology & Biochemistry II

 

CO44:  Study the structure and properties of Macromolecules

CO45:  Understand the biosynthesis of various secondary metabolites.

CO46:  Learn the properties, Enzyme catalysis and activation energy– Mechanism of enzyme action

CO47:  Understand the biosynthesis of tannins, phenols and nitrogenous compounds and their use in day to day lives

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question preparation

 

 

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects
 

 

12.00
Unit I: 
Carbohydrates

Classification, catabolism and anabolism of carbohydrates, Chemistry, conformation and functions of monosaccharides, disaccharides, oligosaccharides, polysaccharides and glycoproteins including starch, cellulose and pectins.                                                                                                                                            

                        

12.00
Unit II: 
Proteins

Structure of protein: primary , secondary ,tertiary, quaternary, Ramachandran plot, techniques of protein purification, ion exchange chromatography, size exclusion chromatography, protein folding, Protein sequencing: chemical and enzymatic hydrolysis of protein to peptides, composition and sequence analysis of proteins: N and C terminal analysis.

Nitrogen Metabolism: Nitrogen cycle, Nitrogen fixation, importance of nitrate reductase and its regulation , Nod factor,  nif and nod genes, glutamate D dehydrogenase reaction

12.00
Unit III: 
Fat Metabolism and Vitamins

Fat metabolism: Introduction, classes of lipids, lipid structure, metabolism of lipids, and function of derived lipids: phospholipids, sphingolipids, cholesterol, plasmalogens and glycerolipids.

Vitamins: Biochemistry and function of thiamine, riboflavin, nicotinic acid, pantothenic acid, pyridoxine, biotin, folic acid, vitamin B12, ascorbic acid, vitamin A , vitamin D vitamin E and  vitamin K, clinical aspect of vitamin deficiency. 

 

12.00
Unit IV: 
Enzymes

Introduction to enzyme, classification, regulation of enzyme activity, mechanism of action, enzyme kinetics, Michaelis-Menten equation, coenzymes, isozymes, ribozymes and abzymes.                  

                                    

12.00
Unit V: 
Secondary metabolites

Biosynthesis and functions of secondary metabolites with special reference to alkaloids, tannins, lignins, sapogenins, coumarins, cardiac glycosides, anthocyanins.            

Essential Readings: 

·Buchanan, BB; Gruissem, W and Jones, RL. 2000. Biochemistry and Molecular Biology of Plants. American Society of Plant Physiologists. Maryland, USA

  • Bugg, TDH. 2004. Introduction of Enzyme and Co-enzyme chemistry (2nd edition). Blackwell Publishing Ltd., UK.
  • Gurr, MI; Harwood, JL and Frayn, KN. Lipid Biochemistry: An Introduction (5th edition). Blackwell Science.
  • Hames, BD and Hooper, NM. 2003. Biochemistry: Instant Notes (2nd edition). Bios Scientific Publishers Ltd.  
  • Lea, PJ and Leegood, RC. 1999. Plant Biochemistry and Molecular Biology. John

            Wiley & Sons, New York.

Lesk, AM. 2001. Introduction to Protein Architechture. Oxford University Press, UK

  • Lydyard, PM; Whelan, A and Fanger, MW. 2003. Instant Notes in Immunology. Hames, BD (ed.). Viva Books Pvt. Ltd., New Delhi
  • Mathews, CK; van Holde, KE and Ahern, KG. 2003. Biochemistry. Pearson Education Pvt. Ltd., New Delhi.
  • Mohr, H and Schopfer, P. 1995. Plant Physiology. Springer- Verlag, Berlin, Germany.
  • Montgomery, R; Conway, TW and Spector, AA. 1990. Biochemistry: A Case Oriented Approach (5th edition). The CV Mosby Co., Torento.
  • Principles and Techniques of Biochemistry and Molecular Biology (6th edition). Wilson, K and Walker, J (eds.). Cambridge University Press
  • Rawn, DJ. Biochemistry. Panima Publishing Co., New Delhi.
  • Sands. 1995. Problems in Plant Physiology. John Murray, London.
  • Srivastava, HN. 2006. Pradeep’s Botany Vol. V. Pradeep Publications, Jalandhar
  • Thimmaiah, SR. 2004. Standard Methods of Biochemical Analysis. Kalyani Publishers.
  • Trehan, K. 1990. Biochemistry (2 nd edition.). New Age International (P) Ltd., Publishers, New Delhi.
  • Voet, D and Voet, J. 1995. Biochemistry (2nd edition). John Wiley & Sons Inc.. New York.
  • West, ES; Todd, WR; Mason, HS and Bruggen, JTV. 1974. Textbook of Biochemistry (4th edition). Oxford & IBH Publishing Co. Pvt. Ltd., New Delhi.
  • Zubay, GL; Parson, WW and Vance, DE. Principles of Biochemistry.

 

 

 

 

Academic Year: 
2019-2020
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