Plant Biotechnology and Bioinformatics

Paper Code: 
BOT 324
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course Objectives:

This course will enable the students to –

  1. To study the various tools and techniques used to improve the plant productivity and to introduce the basic aspects of bioinformatics.
  2. To impart knowledge and practical skills to use biotechnological tools in crop improvement.
  3. To impart knowledge about the methods of molecular separation and characterization
  4. To expose the practical nuances of biotechnology, computing sciences and information tools by way of lab visits.

 

Course Outcomes (COs):

     Course

Course Outcome

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BOT 324

 

Plant Biotechnology and Bioinformatics

On completion of this course, the students will be able to:

CO72:  To understand and summarize different types cyber issues concerning IPR

CO73: To Demonstrate and implement appropriate procedures for obtaining Intellectual Property Protection in India and other countries as well.

CO74:  To Demonstrate and implement the concept of micro propagation through somatic embryogenesis and organogenesis in plants for clonal propagation of elite germplasm.

CO75:  To explain and interpret the basic concepts of Bioinformatics, including computational biology, database design and probability for meaningful results.

CO76: To explain, remember and interpret the specific and non-specific methods of gene transfer for generation of recombinant species.

CO77: To demonstrate and execute protocols related to Recombinant DNA technology and its applications in human welfare

CO78: To explain and implement procedures for developing basic laboratory setup, handling of explant tissue, nutrient medium and establishing the culture and incubation of cultures.

CO79: To remember the fundamentals of bioinformatics tools, computational biology and statistical methods utmost necessary for contemporary research

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question preparation

 

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects
 

 

12.00
Unit I: 
Plant tissue culture

Plant tissue culture: General introduction, history and scope of plant tissue culture, comparision of different plant tissue culture media, concept of totipotency, organogenesis, somatic embryogenesis (direct & indirect), callus culture and suspension culture, somaclonal variation, hardening and acclimatization of plants. 

 Protoplast culture: Isolation and culture of protoplast, somatic hybridization, hybrid selection and regeneration, concept of hybrid and cybrid, achievements and limitations of protoplast culture.

12.00
Unit II: 
Transgenic plants

Transgenic plants: Concept and history of transgenesis in plants, principles and techniques of gene cloning: direct DNA transfer to plant cells, Agrobacterium mediated transformation: Ti plasmid, process of T- DNA transfer and integration, vectors, promoter, terminator, marker and reporter genes, ethical and ecological issues of transgenesis, terminator technology (GURT)

 

12.00
Unit III: 
Intellectual Property Rights:

Intellectual Property Rights: Introduction of IPR, Forms of IPRs, Distinction among various IPRs; Introduction of Paris convention, WIPO, WTO and TRIPS, Indian IP system; Salient features of Patent Act, patentability requirement, prior-art search, Documents  requirement for filing an application for patent, patent publication, technology transfer, Infringements and remedies; Budapest treaty; Protection of plant variety and farmers rights Act, Convention of Biological Diversity, bio-patents, traditional knowledge, case studies – haldi, neem, basmati, GM crops and IPRs, Geographical indicators.

 

12.00
Unit IV: 
Genetic Engineering

Genetic engineering: Molecular tools and their applications: Restriction enzymes,

 C DNA and genomic library, DNA sequencing, polymerase chain reaction, DNA fingerprinting.                                                                                       

 

12.00
Unit V: 
Bioinformatics:

Bioinformatics: Gene bank sequence database, structure database, sequence analysis, information retrieval from biological database, NCBI data model, sequence alignment and database searching and practical aspects of multiple sequence alignment.

 

Essential Readings: 

Essential Readings:

  • Sahni, S., In Prasad, B. D., & In Kumar, P. (2021). Plant Biotechnology: Volume 2. Academic Press, Toronto
  • Prasad, B. D., In Sahni, S., In Kumar, P., & In Siddiqui, M. W. (2021). Plant Biotechnology Volume 1. Academic Press, Toronto
  •  Hill, S. (2016). Analytical Techniques in Biotechnology.  Syrawood Publishing House. United States.
  • S.L.: Syrawood Pub House. Plant Biotechnology: Principles and Applications.
  • Thangadurai, D., Tang, W., & Song, S.-Q. (2007). Plant Stress and Biotechnology. Jaipur, India: Oxford Book Company
  • Slater, A., Scott, N. W., & Fowler, M. R. (2003). Plant Biotechnology. Oxford: Oxford University Press.
  • International Congress of the International Association of Plant Tissue Culture and Biotechnology, & Vasil, I. K. (2011). Plant Biotechnology 2002 and beyond. Dordrecht: Springer.
  • Chawla, H. S. (2000). Introduction to plant biotechnology. Enfield, N.H: Science Publishers.
  • Smith, J. E. (2009). Biotechnology. Cambridge, UK: Cambridge University Press.
  • Ramawat, K. G. (2014). Biotechnology: Secondary metabolites. Enfield : Science
  • Saunders, J., Warmbrodt, R. D., & National Agricultural Library (U.S.). (1993). Biotechnology, plant nutrition: January 1988 - April 1993. Beltsville, Md: National Agricultural Library.
  • Gupta, P. K. (2015). Plant biotechnology. Meerut: Rastogi Publications.
  • Stewart, C.N.. Plant Biotechnology and Genetics: Principles, Techniques, and Applications. (2016). United Kingdom: Wiley.
  • Hopkins, W. G. (2006). Plant Biotechnology. United States: Facts On File, Incorporated.

 

Suggested Readings:

  • Singh, B. D. (2006). Plant Biotechnology. India: Kalayani Publishers.
  • Chrispeels, M. J., Sadava, D. E. (2003). Plants, Genes, and Crop Biotechnology. Italy: Jones and Bartlett Publisher.
  • Brown, T. A. (2016). Gene Cloning and DNA Analysis: An Introduction. Germany: Wiley.
  • Twyman, R., Primrose, S. B. (2013). Principles of Gene Manipulation and Genomics. Germany: Wiley.
  • Krebs, J. E., Kilpatrick, S. T., Goldstein, E. S. (2017). Lewin's GENES XII. Japan: Jones & Bartlett Learning.

 

 

References: 

e-Resources:

·         https://www.apsnet.org/edcenter/disimpactmngmnt/labexercises/PlantBiotechnology/Documents/PlantTissueCulture.pdf

·         https://www.deshbandhucollege.ac.in/pdf/e-resources/bio-chemistry/BCH(H)-VI%20Advanced%20Cell%20Biology-1.pdf

·         http://bioinformaticsinstitute.ru/sites/default/files/lapidus_1_0.pdf

·         https://www.researchgate.net/publication/343039538_Bioinformatics_Concepts_and_Applications

·         https://assets.publishing.service.gov.uk/government/uploads/system/uploads/attachment_data/file/627956/IP-Rights-in-India.pdf

·         https://www.annauniv.edu/ipr/files/downloadable/Overview%20of%20IPR.pdf

·         https://www.researchgate.net/publication/328161728_INTELLECTUAL_PROPERTY_RIGHTS_IPR

·         https://www.gordon.edu/download/pages/Salem-Genetic%20Engineering2003.pdf

·         https://gs.ucdenver.edu/ministem/pdf/2014_miniStem_Bennett.pdf

·         https://nptel.ac.in/courses/102106065 BioInformatics:Algorithms and Applications, IIT Madras

 

Journals:

·         Plant Cell Tissue and Organ Culture. (Springer)

·         Plant Cell Reports (Springer)

·         In Vitro Cellular & Developmental Biology – Plant (Springer)

·         Plant Science (Elsevier)

Plant Biotechnology Journal (Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.)

Academic Year: 
2023-2024
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