Cell and Molecular Biology

Paper Code: 
BOT201
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 
 

Course Objectives: 

This course will enable the students to-

  1. To study the molecular aspects of cell structure and functions in plant cell.
  2. To provide an overview of cell structure and function at the molecular level.
  3. To introduce some of the major ideas and experimental approaches in cell and molecular biology.

Course Outcomes (COs):

Course

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BOT201

 

Cell and Molecular Biology

CO19: Understand the structure of basic components of prokaryotic and eukaryotic cells, especially membranes and organelles.

CO20: Understand how these cellular components are used to generate and utilize energy in cells.

CO21: Understand the cellular components and mechanism of mitotic and meiotic cell division.

CO22: Explain how DNA stores genetic information

CO23: Explain the role of complementary base pairing in the precise replication process of DNA

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question Framing

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects
 

 

 

9.00
Unit I: 
Cell envelopes and organelles

Cell wall: structure, plasma membrane:  structure, models and functions, structure and function of chloroplast, mitochondria, golgi bodies, endoplasmic reticulum, lysosomes, plasmodesmata, vacuoles, ribosomes, biogenesis of chloroplast and mitochondria.

 

9.00
Unit II: 
Nuclear Organization

Ultrastructure of nucleus, nuclear membrane, nucleolus, nuclear pores; Chromosome structure and packaging of DNA, specialized type of chromosome: Polytene and lampbrush chromosome, B chromosomes, Heterochromatin and Euchromatin, Barr bodies

 

9.00
Unit III: 
Cell Division and Cell Cycle

Cell Division: Amitosis, Mitosis, Meiosis.

Cell cycle: Phases of cell cycle, Cell cycle checkpoints, role of cyclins and cyclin dependent kinases.

 

9.00
Unit IV: 
DNA

The Genetic Material: DNA structure, A, B and Z forms; elementary idea of DNA damage and repair, DNA methylation; satellite and repetitive DNA, DNA replication, transcription and translation.

 

9.00
Unit V: 
RNA

The Genetic Material: Biogenesis, structure and its types- rRNA, mRNA, and tRNA, RNA processing (splicing of m RNA, rRNA and tRNA), RNA editing and transposons.

Essential Readings: 
  • Alberts, B; Bray, D; Lewis, J; Raff, M; Roberts, K and Watson, JD. 1999. Molecular Biology of the Cell. Garland Publishing Inc., New York.
  • Buchanan, BB; Gruissem, W and Jones, RL. 2000. Biochemistry and Molecular Biology of Plants. American Society of Plant Physiologists. Maryland, USA.
  • Powar, C.B. Cell Biology. Himalaya Publishing House, Mumbai.
  • Introductory Cell Biology. Veer Bala Rastogi.

 

References: 

·         De Robertis. Cell and Molecular Biology. Waverly International.

·         Karp and Gerald. Cell and Molecular Biology: Concepts and Experiments (3rd edition). John Wiley & Sons.

·         Kleinsmith, LJ and Kish, VM. 1995. Principles of Cell and Molecular Biology (2nd edition). Harper Collins College Publishers. New York, USA.

·         Krishnamurthy, KV. 2000. Methods in Cell Wall Cytochemistry. CRC Press, Boca Raton; Florida.

·         Lewin, B 2000. Genes VII. Oxford University Press, New York.

·         Lodish, H; Berk, A; Zipursky, SL; Matsudiara, P; Baltimore, D and Darnell, J. 2000. Molecular Cell Biology (4th edition). WH Freeman & Co., New York.

·         Rost, T et al. 1998. Plant Biology. Wordsworth Publishing Co. California, USA.

·         Wolfe, SL. 1993. Molecular and Cellular Biology. Wordsworth Publishing Co. California, USA.

Weil, JH. 2003. Molecular Insight in Plant Biology. Oxford & IBH Publishing Co. Pvt. Ltd.

Academic Year: 
2020-2021
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