Advanced Plant Biotechnology I

Paper Code: 
BOT 421A
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to –

  1. To develop an understanding of latest trends and various tools and techniques of Plant Biotechnology for human welfare.
  2. To impart knowledge about the techniques of plant tissue culture
  3.  To study haploid production
  4.  To have a basic understanding of the plant genetic transformation
  5.  To gain knowledge on Cryopreservation
  6.  To know the application of plant tissue culture

 

Course Outcomes (COs):

     Course

Course Outcome

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BOT 421A

 

Elective 1A: Advanced Plant Biotechnology I

On completion of this course, the students will be able to;

CO88:  To focus and recall the core concepts and fundamentals of plant biotechnology, bioreactors and genetic engineering

CO89:  To Demonstrate and explain the competency of different types of plant tissue culture

CO90:  Analyse and interpret the role of different enzymes and vectors used for genetic manipulations

CO91: To Explain gene Cloning and summarize and implement different methods for successful gene transfer in living systems.

CO92: To attribute the major concerns and applications of transgenic technology

CO93:  To interpret and explain the basic concepts of creating a functional Green House and its benefits in the current scenario.

CO94:  To summarize and interpret the significance of secondary metabolites in tissue culture and their commercialization

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question preparation

 

 

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects
 

 

12.00
UNIT I                                                                                               

Plant Tissue Culture: PTC lab, media preparation and handling, cell and plant culture, sterile techniques, totipotency, various plant tissue culture media and their composition. Micropropagation: organogenesis, somatic embryogenesis, axillary bud proliferation.                                                                                                                                           

 

12.00
UNIT II                                                                                              

Recombinant DNA Technology: Cutting and joining DNA molecules, cloning vectors: plasmids, cosmids, bacteriophage vectors, phagemids, YAC, shuttle vectors, expression vectors, gene cloning, direct DNA transfer: particle bombardment, electroporation, liposome mediated transformation, macroinjection; Agrobacterium mediated transformation of plants; in planta gene transfer.

 

12.00
UNIT III                                                                                            

Organ Culture:  Anther culture, mature and immature zygotic embryo culture, ovary culture, somaclonal and gametoclonal variations, triploid production, production of virus free plants. Tissue culture in germplasm conservation: Synthetic seeds, Cryopreservation.                                                                                                                                      

 

12.00
UNIT IV                                                                                             

Greenhouse Hardening Unit:  Greenhouse Construction, types of greenhouses, environmental control inside a greenhouse. Soilless media for propagating and growing nursery plants. Media components: Sand, peat moss, coco-peat, vermiculite, pumice, perlite synthetic plastic aggregate and composts. Preplanting treatments of soil and soil mixes: heat treatments, fumigation and chemicals. 

 

12.00
UNIT V                                                                                              

Secondary metabolite production in culture: Biotransformation, factors affecting production of secondary metabolites, production of important drugs in culture- taxol, ginseng, morphine, codeine, vinblastine and vincristine, types of bioreactors (stirred tank, air lift, membrane type, immobilized cell bioreactors).

 

Essential Readings: 

BOOKS RECOMMENDED:

 

  • Bhojwani, SS and Razdan, MK. 1996. Plant Tissue Culture: Theory and Practice (a revised edition). Elsevier Science Publishers. New York, USA.
  • Brown, TA.2017.Genomes. John Wiley & Sons (Asis) Pvt. Ltd., Singapore.
  • Callow, JA; Ford-Lloyd, BV and Newbury, HJ. 1997. Biotechnology and Plant Genetic Resources: Conservation and Use. CAB International, Oxon, UK.
  • Chawla, HS. 2020. Introduction to Plant Biotechnology. Oxford & IBH Publishing Co. Pvt. Ltd.
  • Primrose, SB. 2002. Principles of Genome Analysis. Blackwell Science Ltd. Oxford, UK.

 

 

Academic Year: 
2021-2022
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