Advanced Plant Biotechnology I

Paper Code: 
BOT 421A
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

The objective of this paper is to study and develop an understanding of latest trends and various tools and techniques of Plant Biotechnology for human welfare.

 

12.00
Unit I: 
Plant Tissue Culture

PTC lab, media preparation and handling, cell and plant culture, sterile techniques, totipotency, various plant tissue culture media and their composition. Micropropagation: organogenesis, somatic embryogenesis, axillary bud proliferation.                     

12.00
Unit II: 
Organ Culture

 

  Anther culture, mature and immature zygotic embryo culture, ovary culture, somaclonal and gametoclonal variations, triploid production, production of virus free plants. Tissue culture in germplasm conservation: Synthetic seeds, Cryopreservation.                                                                                                                                      

12.00
Unit III: 
Greenhouse Hardening Unit

  Greenhouse Construction, types of greenhouses, environmental control inside a greenhouse. Soilless media for propagating and growing nursery plants. Media components: Sand, peat moss, cocopeat, vermiculite, pumice, perlite synthetic plastic aggregate and composts. Preplanting treatments of soil and soil mixes: heat treatments, fumigation and chemicals. 

 

 

12.00
Unit IV: 
Secondary metabolite production in culture

 Biotransformation, factors affecting production of secondary metabolites, production of important drugs in culture- taxol, ginseng, morphine, codeine, vinblastine and vincristine, types of bioreactors (stirred tank, air lift, membrane type, immobilized cell bioreactors).

 

12.00
Unit V: 
Recombinant DNA Technology

 Cutting and joining DNA molecules, cloning vectors: plasmids, cosmids, bacteriophage vectors, phagemids, YAC, shuttle vectors, expression vectors, gene cloning, direct DNA transfer: particle bombardment, electroporation, liposome mediated transformation, macroinjection; Agrobacterium mediated transformation of plants; in planta gene transfer.

                                                                                                                               

Academic Year: