Practicals based on BOT 421A and BOT 422A

Paper Code: 
BOT423
Credits: 
6
Contact Hours: 
180.00
Max. Marks: 
100.00
Objective: 

Course Objective

This course will enable the students to –

  1. To demonstrate procedural knowledge that creates different types of professionals in the field of Botany like in research and development, teaching government and public services. 

 

Course Outcomes (COs):

     Course

Course Outcome

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BOT 423A

 

Practical

CO105:  Learn how to prepare media for tissue culture, isolation of protoplast and organogenesis.

CO106:  Basic concept of protoplast isolation

CO107:  How to demonstrate DNA sequencing

CO108:  Understand basic laboratory setup, handling of explant tissue, nutrient medium and establishing the culture and incubation of cultures.

CO109:  Describe suitable methods for characterizing the activity, function, diversity and composition of microbial commUnities.

CO110:  To demonstrate the ability to identify significant biological research questions, develop research protocols and properly analyze research questions through the use of the scientific method.

Substantial laboratory-based practical component and experiments

Technology-enabled learning

Field-based learning

Project-based learning

Internship in industry, and research establishments.

Literature surveys and evaluations

 

Practical assignments and laboratory reports

Observation of practical skills

Individual and group project reports

 

Viva voce interviews are majorly adopted assessment methods

 

 
 

 

  • Media preparation, sterilization and inoculation of given material.
  • Growth characteristics of E.coli using plating and turbidimetric methods.
  • Isolation of plasmid from E.coli by alkaline lysis method and its quantitation spectrophotometrically.
  • Restriction digestion of the plasmid and estimation of the size of various DNA fragments.
  • Cloning of a DNA fragment in a plasmid vector, transformation of the given bacterial population and selection recombinants.
  • Organogenesis and somatic embryogenesis using appropriate explantsand preparation of artificial seeds.
  • Isolation of protoplast from various plant tissues and testing their viability.
  • Effect of physical and chemical factors protoplat yield.
  • Demonstration of protoplast fusion employing PEG.
  • Demonstration of DNA sequencing using Sanger,s dideoxy method.
  • Demonstration of microtechniques for plant cultures.
  • Co-cultivation of the plant material with Agrobacterium and study GUS activity histochemically.

 

 

 

 

 

References: 
  • Smith and Roberta H. Plant Tissue Culture: Techniques & experiments.
  • Chawla, HS. Plant Biotechnology: Laboratory Manual for Plant Biotechnology.
  • Philips, GC. 1995. Plant Cell, Tissue and Organ Culture: Fundamental Methods. Narosa Publishing House

See the Following Journals

 

  • Plant Cell Tissue and Organ Culture
  • Plant Cell Reports
  • In Vitro Cellular and Developmental Biology- Plant
  • Journal of Experimental Biology
  • Indian Journal of Biochemistry & Biotechnology
  • Phytomorphology
  • Annals of Botany
  • Current Science
  • Sciencia Horticulturae
  • Hortscience
  • Fitoterapia
  • Indian Journal of Biotechnology

 

 

 

 

 

 

 

 

 

 

Academic Year: 
2019-2020
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