Practical

Paper Code: 
BOT 125
Credits: 
9
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course Objective:

This course will enable the students to –

  1. To demonstrate procedural knowledge that creates different types of professionals in the field of Botany like in research and development, teaching government and public services. 

Course Outcomes (COs):

 

     Course

Course Outcome

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BOT 125

 

Practical

 

CO21:  Learn the microscopic technique, familiarize with the external and internal structure of lower group organisms.

CO22:  Students are capable to become practical knowledge about micro-preparation and observation of permanent slides of genera.

CO23:  Learn about the basic concept of DNA isolation, SDS-PAGE and gel electrophoresis.

CO24:  Students are capable to become practical knowledge about micro-preparation and observation of permanent slides of genera.

CO25:  Studied the methods of fossilization and fossil plants

 

Substantial laboratory-based practical component and experiments

Technology-enabled learning

Field-based learning

Project-based learning

 

 

Practical assignments and laboratory reports

Observation of practical skills

Individual and group project reports

 

Viva voce interviews are majorly adopted assessment methods

 

 

 

BOT 121: Cell & Molecular Biology

  • Isolation of RNA from Yeast
  • Restriction Digestion
  • Agarose – Gel Electrophoresis
  • SDS- PAGE
  • Isolation of DNA from plant material

BOT 122: Cytology & Cytogenetics

 

  • Study of general cytological techniques
  • Study of various stages of mitosis in Onion root tips.
  • Study of various stages of meiosis in Onion flower bud
  • Calculation of mitotic index
  • To study B chromosomes in plants
  • Study of pollen viability
  • To measure different types of plant cells using Stage micrometer and Ocular Induction of polyploidy in plants by treating shoot apical meristem with colchicines solution

BOT 123: Hierarchy of Plant Kingdom

  • Preparation of report on Indian contributors in the field of algal, fungal, bryophytic, gymnospermic and angiospermic paleobotany
  • Microscopic examination of stained cell preparation of bacteria by various staining methods
  • Study of role of bacteria in transformation of milk to milk products and in carbohydrate fermentation
  • Determination of Biological Oxygen Demand in pure water and water with algal growth to various degrees
  • Estimation of protein content in Spirulina, the Single Cell Protein
  • Study of mushroom cultivation
  • Study of moisture retaining capacity of peat moss
  • Study of economic importance of Gymnosperms
  • Study of different types of lichen thalli and their role in succession

BOT 124: Microbiology, Fungi & Plant Pathology

  • Cleaning of glassware
  • To study different methods of sterilization and disinfection by physical agents.
  • Instrumentation
  • To study various types of culture media
  • To determine the growth curve in bacteria (E, coli)
  • To study the bacteria by Gram staining
  • To become acquainted with Kirby-Baur procedure
  • Wine production by fermentative activities of Yeast cells
  • Isolation of micro organisms from the mixed culture by spread plate methods
  • Isolation of micro organism by pour plate method
  • To study ecological relationship between micro organisms of soil by Wino Gradsky Column
  • To study the presence of faecal contamination in the given water sample
  • Survey of dermatological diseases caused by Fungi
  • Inventry preparations of antibiotics procured from Fungi
  • Morphological study of representative members of Fungi- Pilobolus, Chaetomium, Morchella, Melampsora, Poly[porus, Dreschlera, Phoma, Peziza, Puccinia
  • Symptomatology, identification, causal organism of the following diseases: White rust, bunt, smut, green ear, smut and ergot of Bajra, rust and smut of wheat, paddy blast, cotton wilt, downy mildew and powdery mildew of grapes, , tundu, tobacco mosaic virus, little leaf of brinjal, root knot of vegetables
  • Report on eminent contributors in Mycology, Plant pathology and important related institutes
Academic Year: