Practical

Paper Code: 
BOT 503
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00

Suggested exercises:

  • Demonstration of phemomenon of plasmolysis using Rheo discolor leaves.
  • Demonstration of phemomenon of osmosis by potato osmoscope.
  • Demonstration of phemomenon of imbibition.
  • Demonstration of phemomenon of root pressure.
  • Demonstration of phemomenon of unequal transpiration in dorsiventral leaves using cobalt chloride paper.
  • Measurement of the rate of transpiration using potometers.
  • Anatomical study of root nodule depicting symbiotic relationship between nitrogen fixing bacteria and roots of leguminuous plants.
  • To demonstrate that light, CO2 and chlorophyll are necessary for photosynthesis.
  • To demonstrate that O2 is evolved during photosynthesis.
  • To determine the value of RQ of different respiratory substrates by Ganong’s respiroscope.
  • To demonstrate that CO2 is given out during respiration
  • Measurement of growth by arc auxanometer.
  • Phytochemical tests for the following- glucose, starch, proteins, fats, tannins, ascorbic acid and anthocyanins.
  • Demonstration of respiratory enzymes- peroxidase, catalase and dehydrogenase in plant tissue samples and measurement of their activities.

 

 

References: 
  • Ramakrishnan, S; Prasannan, KG and Rajan, R. Textbook of Medical Biochemistry (2nd edition). Orient Longman.
  • Methods in Plant Biochemistry and Molecular Biology. 1997. Dashek, WV (ed.). CRC Press.
  • Wilson and Walker .Practical Biochemistry: Principles and Techniques. Cambridge University Press. U.K.

 

Academic Year: