Plant Physiology and Biochemistry II

Paper Code: 
25BOT224
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to –

  1. To study the various biochemical pathways in plants and to study the structure of various biomolecules.
  2. To enhance the knowledge on physiology and biochemical aspects through a series of experiments
  3. To disseminate facts on the fundamental aspects of biochemical interactions specific to plants

 

Course Outcomes: 

Course

 Course Outcomes

Learning and teaching strategies

Assessment Strategies

Course Code

Course Title

25BOT224

      

Plant Physiology and Biochemistry II

 

CO51: Investigate and apply the knowledge of structure, confirmation, chemistry and catabolism of carbohydrates

CO52: Build knowledge about the structure function of proteins, and compile various techniques of protein purification. Discuss nitrogen metabolism in detail and also elaborate its importance and regulation.

CO53: Develop an understanding of Fat metabolism and elaborate and compile the biochemistry and functions of vitamins.

CO54: Assess the structure and mechanism of enzyme action for developing a better understanding of enzyme kinetics.

CO55: Outline the biosynthetic pathway of secondary metabolites and summarize their functions. Propose research hypothesis on secondary metabolites and its role in pharmaceuticals.

CO56: Contribute effectively in course-specific interaction.

Approach in Teaching:

Interactive-

Lectures

Tutorials

Discussion

Demonstration

Learning Activities for the students:

Peer learning

Seminars, presentations, model preparations, graphical representations

 

 

 

Class test, Semester end examinations, Quiz, Presentations, Flipped classrooms, Interactive sessions

 

12.00
Unit I: 
Carbohydrates
Carbohydrates: Classification, catabolism and anabolism of carbohydrates, Chemistry, conformation and functions of monosaccharides, disaccharides, oligosaccharides, polysaccharides and glycoproteins including starch, cellulose and pectins.                                                                                                                                            

 

12.00
Unit II: 
Proteins & Nitrogen Metabolism
Proteins: Structure of protein: primary, secondary, tertiary, quaternary, Ramachandran plot, techniques of protein purification, protein folding, Protein sequencing: chemical and enzymatic hydrolysis of protein to peptides, composition and sequence analysis of proteins: N and C terminal analysis.

Nitrogen Metabolism: Nitrogen cycle, Nitrogen fixation, importance of nitrate reductase and its regulation, Nod factor, nif and nod genes, glutamate D dehydrogenase reaction

12.00
Unit III: 
Fat & Vitamins

Fat metabolism: Introduction, classes of lipids, lipid structure, metabolism of lipids, and function of derived lipids: phospholipids, sphingolipids, cholesterol, plasmalogens and glycerolipids.

Vitamins: Biochemistry and function of thiamine, riboflavin, nicotinic acid, pantothenic acid, pyridoxine, biotin, folic acid, vitamin B12, ascorbic acid, vitamin A, vitamin D vitamin E and vitamin K, clinical aspect of vitamin deficiency. 

 

12.00
Unit IV: 
Enzymes

Enzymes: Introduction to enzyme, classification, regulation of enzyme activity, mechanism of action, enzyme kinetics, Michaelis-Menten equation, coenzymes, isozymes, ribozymes and abzymes.  

 

 

12.00
Unit V: 
Secondary Metabolites

Secondary metabolites: Biosynthesis and functions of secondary metabolites with special reference to alkaloids, tannins, lignins, sapogenins, coumarins, cardiac glycosides, anthocyanins.           

 

 

Essential Readings: 

Essential Readings:

  • Berg, J. M., Tymoczko, J. L., Stryer, L., Gatto, G. J. (2019). Biochemistry. United States: W.H. Freeman/McMillan Learning.
  • Buchanan, B.B; Gruissem, W and Jones, R.L. (2000). Biochemistry and Molecular Biology of Plants. American Society of Plant Physiologists. Maryland, USA
  • Srivastava, H.N. (2006). Pradeep’s Botany Vol. V. Pradeep Publications, Jalandhar
  • Voet, D. and Voet, J. (2010). Biochemistry (2nd edition). John Wiley & Sons Inc. New York.
  • Mohr, H. and Schopfer, P. 1995. Plant Physiology. Springer- Verlag, Berlin, Germany.
  • Lehninger. (2017). Principles of Biochemistry. Wadsworth Publishing Co. California, USA.
  • Hegde, P.H. (2005). Text Book of Biochemistry. Mohit Publications, New Delhi.
  • Lea, P.J. and Leegood, R.C.1999.Plant Biochemistry and Molecular Biology. John Wiley & Sons, New York.
  • Lesk, A.M. (2010). Introduction to Protein Architecture. Oxford University Press, UK
  • Mathews, C.K; van Holde, K.E and Ahern, K.G. (2003). Biochemistry. Pearson Education Pvt. Ltd., New Delhi.
  • Montgomery, R; Conway, T.W and Spector, A.A. 1996. Biochemistry: A Case Oriented Approach (5th edition). The CV Mosby Co., Toronto.

 

Suggested Readings:

  • Wilson, K. and Walker, J. (Eds.) (2010). Principles and Techniques of Biochemistry and Molecular Biology (6th edition). Cambridge University Press
  • Sands, M. K. (1975). Problems in Plant Physiology. United Kingdom: Murray.
  • Thimmaiah, S.R. (2004). Standard Methods of Biochemical Analysis. Kalyani Publishers.
  • Trehan, K. (1990). Biochemistry (2nd edition.). New Age International (P) Ltd., Publishers, New Delhi.
  • West, E.S., Todd, W.R., Mason, H.S. and Bruggen, J.T.V. (1974). Textbook of Biochemistry (4th edition). Oxford & IBH Publishing Co. Pvt. Ltd., New Delhi.
  • Zubay, G.L.; Parson, W.W. and Vance, D.E. (1995). Principles of Biochemistry.
  • Jain, J. L., Jain, S., Jain, N., (2016). Cell Biology (Cytology, Biomolecules and Molecular Biology).  India: S CHAND & Company Limited.

 

 

References: 

e-Recourses:

 

Journals:

  • Annual Review of Biochemistry (Annual Reviews, US)
  • Biochemistry and Cell Biology (Canadian Science Publishing)
  • Journal of Biochemistry (Oxford University Press)
  • Journal of Plant Biochemistry and Biotechnology (Springer, India)
  • Journal of Experimental Plant Biology (Oxford University Press)
  • Journal of Experimental Botany (Oxford University Press)

 

 

Academic Year: 
2025-2026
  • Printer-friendly version
  • PDF version