PLANT BIOTECHNOLOGY

Paper Code: 
24DBOT601A
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to-

  1. To study the tools and techniques of plant biotechnology and advanced microscopy.
  2. To make the students gain knowledge on methods of molecular separation and characterization
  3. To impart knowledge and practical skills to use biotechnological tools in crop improvement.
  4. To equip the students with knowledge of intellectual property rights (IPR) related protection systems and their significance.

 

 

Course Outcomes: 

Course

Learning Outcome

(at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course title

24DBOT

601A

 

 

PLANT BIOTECHN-OLOGY

 

CO51: Apply and analyze the role of vectors and restriction enzymes in genetic engineering.

CO52: Apply the knowledge of recombinant DNA technology for developing transgenics with desired characters and encourage innovation and creativity while protecting the rights of creators.

CO53: Learn various techniques like electrophoresis, chromatography, PCR and design research experiments related to identification, characterization, amplification and separations of biomolecules.

CO54: Understand the working and principle of techniques like microscopy, centrifugation, flow cytometry and spectrometry and apply them in biotechnological experiments.

CO55: Learn the basic aspects of plant tissue culture and apply their knowledge in creating artificial seeds, somatic hybrids, cybrids and haploid plants for conservation of elite germplasm.

CO56: Contribute effectively in course-specific interaction.

 

Approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

Learning activities for the student:

Self-learning assignment, Effective questions, Stimulation, Seminar presentation

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects
 

 

12.00
Unit I: 
Genetic engineering I

Restriction endonucleases, cutting and joining of DNA molecules, cloning vectors: plasmids, cosmids, bacteriophages, phagemids and YAC. Genomic and cDNA library.

 

 

12.00
Unit II: 
Genetic engineering II

Gene transfer by Agrobacterium, transgenic crops- Bt cotton, Golden rice, Flavr savr tomato, edible vaccines, Intellectual Property Rights (IPR).

 

 

12.00
Unit III: 
Tools and Techniques I

Gel electrophoresis, Agarose gel electrophoresis, Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS PAGE), High Performance Liquid Chromatography (HPLC), Polymerase Chain Reaction (PCR), Enzyme Linked Immunosorbent Assay (ELISA)

 

12.00
Unit IV: 
Tools and Techniques II

Flow cytometry, ultracentrifugation, FISH (Fluorescent in situ hybridization), GISH (Genomic in situ hybridization), SEM (Scanning Eelectron   Microscopy), TEM (Transmission   Electron Microscopy), mass spectrometry.

 

12.00
Unit V: 
Plant Tissue Culture

Basic aspects of plant tissue culture, Cellular totipotency, micropropagation and organogenesis, somatic embryogeneis, artificial seeds, Isolation and culture of protoplast, somatic hybridization and cybridization, anther culture.

 

Essential Readings: 
  • Bhojwani, S.S. and Razdan, M.K. (2012). Plant Tissue Culture: Applications and Limitations. Netherlands: Elsevier Science.
  • Chawla, H.S. (2011). Introduction to Plant Biotechnology. Oxford & IBH Publishing Co. Pvt. Ltd.
  • H.K. Jain, M.C. Kharkwal. (2012). Plant Breeding: Mendelian to Molecular Approaches.  India: Springer Netherlands.
  • Kartha, K.K. (1985). Cryopreservation of Plant Cells and Organs.
  • Bajaj, Y. P. S. (2010). Cryopreservation of Plant Germplasm I. Germany: Springer Berlin Heidelberg.
  • Vasil I.K., Thorpe T.A. (2010). Plant Cell and Tissue Culture.  Netherlands: Springer Netherlands.
  • Narayanaswamy, S. (1994). Plant Cell and Tissue Culture. India: Tata McGraw-Hill Publishing Company.
  • Shantharam, S. and Montgomery, J.F. (1999). Biotechnology, Biosafety and Biodiversity. Oxford & IBH Publishing Co. Pvt. Ltd., New Delhi

 

 

References: 
  • Brown, T. A. (2018). Genomes 4. United States: CRC Press.
  • Primrose, S.B. (2013). Principles of Genome Analysis. Blackwell Science Ltd. Oxford, UK.
  • Gregory C. P., Gamborg O. (2013). Plant Cell, Tissue and Organ Culture: Fundamental Methods. Germany: Springer Berlin Heidelberg.
  • Callow, J.A., Ford-Lloyd, B.V. and Newbury, H.J. (1997). Biotechnology and Plant Genetic Resources. Conservation and Use. CAB International, Oxon, UK.
  • Rédei, G. P. (1998). Genetics Manual: Current Theory, Concepts, Terms. Singapore: World Scientific.
  • Collins, H.A. and Edwards, S. (1998). Plant Cell Culture. Bios Scientific Publishers. Oxford, UK.
  • Gustafson, J.P. (2000). Genomes. Kluwer Academic Plenum Publishers. New York.
  • Jolles, O. and Jomvall, H. (eds.). (2000). Proteomics in Functional Genomics. Birkhauser Verlag, Basel, Switzerland.
  • Malkoff C. (2016). Functional Genomics and Proteomics. United States: Syrawood Publishing House.
  • Edwards and Sue. (1998). Plant Cell Culture. Bios Scientific Publishers, London.
  • Ignacimuthu, S. (2001). Plant Biotechnology. Oxford & IBH Publishing Co. Pvt. Ltd.

 

e-Resources:

Journals:

  • Plant Biotechnology Journal (Wiley Online Library)
  • Plant Biotechnology Reports (Springer)
  • Journal of Plant Biochemistry and Biotechnology (Springer)
  • Plant Biotechnology (Hindwai)
  • Plant Biotechnology (Frontiers in Plant Science)

 

 

 

 

 

Academic Year: 
2024-2025
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