Plant Biotechnology

Paper Code: 
BOT 601
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 

To study the tools and techniques of plant biotechnology .

 

9.00
Unit I: 
Tools and Techniques I

Gel electrophoresis, Agarose gel electrophoresis, Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS PAGE), High Performance Liquid Chromatography (HPLC), Polymerase Chain Reaction (PCR), EnzymeLlinked Immunosorbent Assay (ELISA)

             

 

 

Unit II: 
Tools and Techniques II

Flow cytometry,  ultracentrifugation, FISH (Fluorescent in situ hybridization), GISH (Genomic in situ hybridization), SEM (Scanning  Eelectron   Microscopy), TEM (Transmission   Electron Microscopy), mass spectrometry.

                             

9.00
Unit III: 
Plant tissue culture

 Basic aspects of plant tissue culture, Cellular totipotency, micropropagation and organogenesis,  somatic embryogeneis, artificial seeds, Isolation and culture of protoplast, somatic hybridization and cybridization, anther culture.

 

                                                                                                                                                                                                                                                                       

 

  .

                                                                                                                                                                                                                                                                                                                                                                                                                   

 

 

  

                                                                                                                       

 

9.00
Unit IV: 
Genetic engineering I

Restriction endonucleases, cutting and joining of DNA molecules, cloning vectors: plasmids, cosmids, bacteriophages, phagemids and YAC. Genomic and cDNA library

9.00
Unit V: 
Genetic engineering II

Gene transfer by Agrobacterium, transgenic crops- Bt cotton, Golden rice, Flavr savr tomato, edible vaccines, Intellectual Property Rights (IPR).

Essential Readings: 

Essential Books for BOT 601

 

  • Bhojwani, SS and Razdan, MK. 1996. Plant Tissue Culture: Theory and Practice (a revised edition). Elsevier Science Publishers. New York, USA.
  • Brown, TA.1999.Genomes. John Wiley & Sons (Asis) Pvt. Ltd., Singapore.
  • Callow, JA; Ford-Lloyd, BV and Newbury, HJ. 1997. Biotechnology and Plant Genetic Resources: Conservation and Use. CAB International, Oxon, UK.
  • Chawla, HS. 2002. Introduction to Plant Biotechnology. Oxford & IBH Publishing Co. Pvt. Ltd.
  • Primrose, SB. 1995. Principles of Genome Analysis. Blackwell Science Ltd. Oxford, UK.

 

 

References: 
  • Chrispeels, MJ and Sadava, DE. 1994. Plants, Genes and Agriculture. Jones and Barlett Publishers. Boston, USA.
  • Collins, HA and Edwards, S. 1998. Plant Cell Culture. Bios Scientific Publishers. Oxford, UK.
  • Gustafson, JP. 2000. Genomes. Kluwer Academic Plenum Publishers. New York.
  • Jolles, O and Jomvall, H(eds.) .2000. Proteomics in Functional Genomics. Birkhauser Verlag, Basel, Switzerland.
  • Kartha, KK. 1985. Cryopreservation of Plant Cells and Organs.
  • Edwards and Sue. 1998. Plant Cell Culture. Bios Scientific Publishers, London.
  • Ignacimuthu, S. 2001. Plant Biotechnology. Oxford & IBH Publishing Co. Pvt. Ltd.
  • Murphy and Terence, M. 1998. Plant Biology. Wadsworth Publishing Co., California.
  • Narayanswamy. 1994. Plant Cell and Tissue Culture. Tata McGraw Hill , New Delhi.
  • Shantharam, S and Montgomery, JF. 1999. Biotechnology, Biosafety and Biodiversity. Oxford & IBH Publishing Co. Pvt. Ltd., New Delhi
  • Singh and Rita. 2004. Plant Biotechnology. Global Vision Publishing House.
  • Vasil, IK and Thorpe, TA. 1994. Plant Cell and Tissue Culture. Kluwer Academic Publishers, The Netherlands

 

 
Academic Year: 
2016-2017
  • Printer-friendly version
  • PDF version