Practical

Paper Code: 
BOT 503
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to-

  1. To develop skills related to qualitative and quantitative estimations of biochemical

Course Outcomes (COs):

Course

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BOT 503

 

Practical

At the end of the course the students will be able to:

CO77:  Acquire knowledge on the physiological functions of plants.

CO78: Basic understanding of the physiological mechanisms of plants.

CO79:  Acquire a general knowledge of the physical, chemical properties and metabolism of carbohydrates and lipids in living system.

CO80: Apply basic knowledge of biomolecules such as carbohydrates, lipids, protein, nucleic acid and enzymes and physiological functions for entrepreneurial development.

CO81:  Plan the experiments efficiently keeping in view the fundamental biochemical principles of enzymes in living system.

 

Substantial laboratory-based practical component and experiments

 

Technology-enabled learning

 

Field-based learning

Project-based learning

 

Practical assignments and laboratory reports

Observation of practical skills

Individual and group project reports

Viva voce interviews are majorly adopted assessment methods

 

 
 

 

  • Demonstration of phemomenon of plasmolysis using Rheo discolor leaves.
  • Demonstration of phemomenon of osmosis by potato osmoscope.
  • Demonstration of phemomenon of imbibition.
  • Demonstration of phemomenon of root pressure.
  • Demonstration of phemomenon of unequal transpiration in dorsiventral leaves using cobalt chloride paper.
  • Measurement of the rate of transpiration using potometers.
  • Anatomical study of root nodule depicting symbiotic relationship between nitrogen fixing bacteria and roots of leguminuous plants.
  • To demonstrate that light, CO2 and chlorophyll are necessary for photosynthesis.
  • To demonstrate that O2 is evolved during photosynthesis.
  • To determine the value of RQ of different respiratory substrates by Ganong’s respiroscope.
  • To demonstrate that CO2 is given out during respiration
  • Measurement of growth by arc auxanometer.
  • Phytochemical tests for the following- glucose, starch, proteins, fats, tannins, ascorbic acid and anthocyanins.
  • Demonstration of respiratory enzymes- peroxidase, catalase and dehydrogenase in plant tissue samples and measurement of their activities.Ramakrishnan, S; Prasannan, KG and Rajan, R. Textbook of Medical Biochemistry (2nd edition). Orient Longman.
  • Methods in Plant Biochemistry and Molecular Biology. 1997. Dashek, WV (Ed.). CRC Press.
  • Wilson and Walker .Practical Biochemistry: Principles and Techniques. Cambridge University Press.U.K.

 

 

 

Essential Readings: 
  • Ramakrishnan, S; Prasannan, KG and Rajan, R. Textbook of Medical Biochemistry (2nd edition). Orient Longman.
  • Methods in Plant Biochemistry and Molecular Biology. 1997. Dashek, WV (Ed.). CRC Press.
  • Wilson and Walker .Practical Biochemistry: Principles and Techniques. Cambridge University Press.U.K.

 

Academic Year: 
2021-2022
  • Printer-friendly version
  • PDF version