Practical based on BOT 601 and 602

Suggested Exercises:

•   Study of laboratory requirements and general techniques of biotechnology.

•   Principle and application of the following- Laminar Air Flow, Autoclave and Ultracentrifugation.

•   Preparation of different types of tissue culture medias and solutions of nutrients and growth regulators

•   Demonstration of inoculation technique, asceptic transfer of explant, optimizing the conditions for direct/ indirect differentiation in different explants to study totipotency, cytodifferentiation and organogenesis.

•   Germination of pollen of Catharanthus roseus in 10 % sucrose

      solution.

•    Demonstration of the technique of protoplast isolation and somatic embryogenesis in Daucus carota.

•   Demonstration of the technique of anther culture in Datura.

•   Demonstration of the technique of gel electrophoresis of bacterial DNA.

•   Demonstration of the phenomenon of action of Restriction Endonucleases.

•    Field study of various plant forms- climbers, trees, shrubs, orchids,

      ferns and palms.

•   Experimental study of the technique of vegetative propagation-cutting, budding, grafting and air layering etc.

•   To study propagation by means of bulbs, corms, tubers, rhizome etc.

•   To study different styles of garden design.

•   Field visit to understand different patterns of landscape design.

•   Preparation of record on local biodiversity.