Practicals based on BOT 601 & BOT 602

Suggested Exercises:

• Study of laboratory requirements and general techniques of biotechnology.
• Principle and application of the following- Laminar Air Flow, Autoclave and Ultracentrifugation.
• Preparation of different types of tissue culture medias and solutions of nutrients and growth regulators
• Demonstration of inoculation technique, asceptic transfer of explant, optimizing the conditions for direct/ indirect differentiation in different explants to study totipotency, cytodifferentiation and organogenesis.
• Germination of pollen of Catharanthus roseus in 10 % sucrose  solution.
• Demonstration of the technique of protoplast isolation and somatic embryogenesis in Daucus carota.
• Demonstration of the technique of anther culture in Datura.
• Demonstration of the technique of gel electrophoresis of bacterial DNA.
• Demonstration of the phenomenon of action of Restriction Endonucleases.
• Field study of various plant forms- climbers, trees, shrubs, orchids, ferns and palms.
• ue of vegetative propagation-cutting, budding, grafting and air layering etc.
• To study propagation by means of bulbs, corms, tubers, rhizome etc.
• To study different styles of garden design.
• Field visit to understand different patterns of landscape design.
• Preparation of record on local biodiversity.