Practicals based on BOT 501 & BOT 502

Paper Code:

BOT503

Credits:

Contact Hours:

60.00

Max. Marks:

100.00

Objective:

Suggested exercises:

Demonstration of phemomenon of plasmolysis using Rheo discolor leaves.
Demonstration of phemomenon of osmosis by potato osmoscope.
Demonstration of phemomenon of imbibition.
Demonstration of phemomenon of root pressure.
Demonstration of phemomenon of unequal transpiration in dorsiventral leaves using cobalt chloride paper.
Measurement of the rate of transpiration using potometers.
Anatomical study of root nodule depicting symbiotic relationship between nitrogen fixing bacteria and roots of leguminuous plants.
To demonstrate that light, CO₂ and chlorophyll are necessary for photosynthesis.
To demonstrate that O₂ is evolved during photosynthesis.
To determine the value of RQ of different respiratory substrates by Ganong’s respiroscope.
To demonstrate that CO₂is given out during respiration
Measurement of growth by arc auxanometer.
Phytochemical tests for the following- glucose, starch, proteins, fats, tannins, ascorbic acid and anthocyanins.
Demonstration of respiratory enzymes- peroxidase, catalase and dehydrogenase in plant tissue samples and measurement of their activities.

Essential Readings:

· Ramakrishnan, S; Prasannan, KG and Rajan, R. Textbook of Medical Biochemistry (2^nd edition). Orient Longman.

· Methods in Plant Biochemistry and Molecular Biology. 1997. Dashek, WV (ed.). CRC Press.

· Wilson and Walker .Practical Biochemistry: Principles and Techniques. Cambridge University Press.U.K

Academic Year:

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