Practicals based on BOT 121, BOT 122, BOT 123 & BOT 124

Paper Code: 
BOT125
Credits: 
8
Contact Hours: 
240.00
Max. Marks: 
100.00
Objective: 

To give insight into scientific knowledge and develop  expertise in these.

BOT125

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

  1. Learn the microscopic technique, familiarize with the external and internal structure of lower group organisms.
  2. Students are capable to become practical knowledge about micro-preparation and observation of permanent slides of genera.
  3. Learn about the basic concept of DNA isolation, SDS-PAGE and gel electrophoresis.
  4. Students are capable to become practical knowledge about micro-preparation and observation of permanent slides of genera.
  5. Studied the methods of fossilization and fossil plants

 

Substantial laboratory-based practical component and experiments

Technology-enabled learning

Field-based learning

Project-based learning

 

Practical assignments and laboratory reports

Observation of practical skills

Individual and group project reports

 

Viva voce interviews are majorly adopted assessment methods

 

 

 

 

Unit I: 
Practical exercises of Bot 121
  • Isolation of RNA from Yeast
  • Restriction Digestion
  • Agarose – Gel Electrophoresis
  • SDS- PAGE
  • Isolation of DNA from plant material

 

 

Unit II: 
Practical exercises of Bot 122
  • Study of general cytological techniques
  • Study of various stages of mitosis in Onion root tips
  • Study of various stages of meiosis in Onion flower bud
  • Calculation of mitotic index
  • To study B chromosomes in plants
  • Study of pollen viability
  • To measure different types of plant cells using Stage micrometer                and Ocular micrometer
  • Induction of polyploidy in plants by treating shoot apical meristem with colchicines solution

 

 

Unit III: 
Practical exercises of Bot 123
  • Preparation of report on Indian contributors in the field of algal, fungal, bryophytic, gymnospermic and angiospermic paleobotany
  • Microscopic examination of stained cell preparation of bacteria by various staining methods
  • Study of role of bacteria in transformation of milk to milk products and in carbohydrate fermentation
  • Determination of Biological Oxygen Demand in pure water and water with algal growth to various degrees
  • Estimation of protein content in Spirulina, the Single Cell Protein
  • Study of mushroom cultivation
  • Study of moisture retaining capacity of peat moss
  • Study of economic importance of Gymnosperms
  • Study of different types of lichen thalli and their role in succession

 

 

Unit IV: 
Practical exercises of Bot 124
  • Cleaning of glassware
  • To study different methods of sterilization and disinfection by physical agents.
  • Instrumentation
  • To study various types of culture media
  • To determine the growth curve in bacteria (E.coli)
  • To study the bacteria by Gram staining
  • To become acquainted with Kirby-Baur procedure
  • Wine production by fermentative activities of Yeast cells
  • Isolation of micro organisms from the mixed culture by spread plate methods
  • Isolation of micro organism by pour plate method
  • To study ecological relationship between micro organisms of soil by Wino Gradsky Column
  • To study the presence of faecal contamination in the given water sample
  • Survey of dermatological diseases caused by Fungi
  • Inventry preparations of antibiotics procured from Fungi
  • Morphological study of representative members of Fungi- Pilobolus, Chaetomium, Morchella, Melampsora, Polyporus, Dreschlera, Phoma, Peziza, Puccinia
  • Symptomatology, identification, causal organism of the following diseases: White rust, bunt, smut, green ear, smut and ergot of Bajra, rust and smut of wheat, paddy blast, cotton wilt, downy mildew and powdery mildew of grapes, tundu, tobacco mosaic virus, little leaf of brinjal, root knot of vegetables
  • Report on eminent contributors in Mycology, Plant pathology and important related institutes

 

 

References: 
  • Khasim, SM. 2002. Botanical Microtechnique: Principles and Practice. Capital   
  • Publishing Co., New Delhi.
  • Protein Expression: A Practical Approach. Ed. Higgins, SJ and Hames, BD.
  • Oxford University Press
  • Malacinski, GM and Freifelder, D. Essentials of Molecular Biology III Ed.Jones & Barlett Publishers.
  • ·Pandey, BP and Trivedi, PC. 1997. Botany Vol. I(10th edition). Vikas Publishing House.
  • Pandey, BP; Misra; Trivedi, PC. 1997. Botany Vol. II. Vikas Publishing House.
  • Pandey, BP and Chadha. 1997. Botany Vol. III. Vikas Publishing House
  • ·         Pandey, BP and Trivedi, PS. 1997. Botany Vol. I(10th edition). Vikas Publishing House.
    ·         Pandey, BP,Pandey, BP; Misra; Trivedi, PS. 1997. Botany Vol. II. Vikas Publishing House.
    ·         Pandey, BP and Chadha. 1997. Botany Vol. III. Vikas Publishing House.
    ·         Santra, SC and Chatterjee. 2005. College Botany Practical Vol. I. New Central  Book Agency (P) Ltd.  

    Kumar, S and Kashyap. 2003. Manual of Practical Algae. Campus Books International, New Delhi               

  • Santra, SC and Chatterjee. 2005. College Botany Practical Vol. I. New Central Book Agency (P) Ltd.,Calcutta.
  • Santra, SC. 2005. College Botany Practical Vol. II. New Central Book Agency (P) Ltd.

  •      

 

 

Academic Year: 
2019-2020
  • Printer-friendly version
  • PDF version