Practical

Paper Code: 
DBOT 806
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
1. To demonstrate practices and procedures that lead to acquisition of skills required for
creating different types of professionals in the field of Botany like in research and
development, teaching government and public services.
Course Outcomes (COs):
Course Outcome Learning
and
teaching
strategies
Assessment
Strategies
On completion of this course, the
students will be able to:
CO116: Acquire skills to extract and
isolate secondary metabolites from plants
and create biologically active products.
CO117: Develop hands on skills and
techniques related to plant physiology so
that they can design their own
experiments
CO118: Acquire knowledge on the
physiological functions of plants.
CO119: Acquire basic knowledge of the
biological importance of the
phytochemicals such as alkaloids, tannins,
anthocyanins, etc.
CONTENTS:
 Demonstration of phenomenon of osmosis by using potato osmometer.
 Demonstration of phenomenon of imbibition.
 Demonstration of the stomatal transpiration by four leaves method.
 To demonstrate that oxygen is evolved during photosynthesis by inverted funnel
method.
 To demonstrate that CO2, water, light and chlorophyll are essential for photosynthesis
by ‘Moll’s half leaf’ experiment.
 To determine the chlorophyll a / chlorophyll b ratio in C3 and C4 plants.
 Isolation of intact chloroplasts and estimation of chloroplast proteins by spot protein
assay.
 Extraction of chloroplast pigments from leaves and preparation of the absorption
spectrum of chlorophylls and carotenoids.
 To demonstrate photophosphorylation in intact chloroplasts, resolve the
phosphoproteins by SDS-PAGE and perform autoradiography.
 To determine the value of RQ of different respiratory substrates using Ganong’s
respirometer.
 Demonstration of respiratory enzymes in plant tissues.
 Demonstration of the substrate inducibility of the enzyme nitrate reductase.
 Measurement of growth using auxanometer.
 To study the effect of plant growth regulators on plant growth.
 To separate the given mixture of dyes using paper chromatography.
 To separate the given mixture of amino acids using thin - layer chromatography.
 To separate the given mixture of pigments using thin – layer chromatography.
 Principles of colorimetry and spectrophotometry.
 Tests for – Alkaloids, Terpenes, Tannins and Anthocyanins

 

Essential Readings: 
Books Recommended:
 Ramakrishnan, S., Prasannan, K. G., & Rajan, R. (2001). Textbook of medical
biochemistry. Hyderabad: Orient Longman.
 Plummer, D. T. (2001). Introduction to Practical Biochemistry. India: Tata McGraw Hill
Publishing Company..
 Singh, S.P.(2007) Practical Manual of Biochemistry. CBS Publisher, New Delhi.
 Bajracharya, D. (2003). Experiments in plant physiology: A laboratory manual. New
Delhi: Narosa.
 Santra, S.C. (2015). College Botany Practical Vol.II. New Central Book Agency (P)
Ltd., Calcutta.
 Bendre, A.M. (2009). A Text Book of Practical Botany. Rastogi Publications, Meerut.
 Pandey,S.N., Misra, S.P., Trivedi, P.C. (2016). Botany Vol. II. Vikas Publishing House.
 Pandey,S.N., Misra, S.P., Trivedi, P.C. (2018). Botany Vol. III. Vikas Publishing House
e-Resources:
Biomolecules
Resources
Function in Health and Disease
Microbiology
Academic Year: 
2023-2024
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