Practical

Paper Code: 
DBOT 502A
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to-

  1. To develop skills related to qualitative and quantitative estimations of biomolecules

 

Course Outcomes (COs):

Course Outcomes

Learning and teaching strategies

Assessment Strategies

 
 

At the end of the course the students will be able to:

CO42:  Acquire a general knowledge of the properties and metabolism of carbohydrates, proteins and lipids in living system.

CO43: Apply basic knowledge of biomolecules such as carbohydrates, lipids, protein, and enzymes and physiological functions for entrepreneurial development.

CO44:  Plan the experiments efficiently keeping in view the fundamental biochemical principles of enzymes in living system.

Substantial laboratory-based practical component and experiments

 

Technology-enabled learning

 

Field-based learning

Project-based learning

 

Practical assignments and laboratory reports

Observation of practical skills

Individual and group project reports

Viva voce interviews are majorly adopted assessment methods

 
 

 

  • Phytochemical tests for the following- glucose, starch, proteins, fats, tannins, ascorbic acid and anthocyanins.
  • Demonstration of enzyme activity- peroxidase, catalase and dehydrogenase in plant tissue samples and measurement of their activities.
  • Quantitative Estimation of protein content by Folin – Lowry method
  • Quantitative Estimation of protein content by Biuret Reagent method.
  • Anatomical study of root nodule depicting symbiotic relationship between nitrogen fixing bacteria and roots of leguminuous plants.
  • Separation of plant pigments & dyes using chromatographic techniques (paper, TLC)
  • Separation of amino acids using chromatographic techniques (paper, TLC).
  • Qualitative and quantitative estimation of secondary metabolites as prescribed in the syllabus using Soxhlet apparatus.

 

Essential Readings: 
  • Dashek, W. V. (2018). Methods in plant biochemistry and molecular biology. CRC Press.
  • Wilson, K., Wilson, K., & Walker, J. (2005). Practical Biochemistry: Principles and Techniques. U.K: Press Syndicate of the University of Cambridge.
  • Bendre, A. (2020). Practical botany: Plant physiology, biochemistry, biotechnology, ecology and plant utilization. Meerut, India: Rastogi Publications.
  • Santra, S. C. (2020). Practical Botany. La Vergne: New Central Book Agency.
  • Harborne, J. B. (2012). Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis, 3E. India: Springer.
  • Damodaran, G. K., K, G. D. (2016). Practical Biochemistry. India: Jaypee Brothers Medical Publishers Pvt. Lited.
  • Soundravally, A., Dhiman, P., R, (2019). Biochemistry Practical Manual E-Book. books.google.co.in › books
  • Wood, E. J. (2012). Practical Biochemistry for Colleges. United Kingdom: Elsevier Science.
  •  Sawhney, S. K. (2005). Introductory Practical Biochemistry. Unitedju Kingdom: Alpha Science International.
  • Ramakrishnan, S; Prasannan, KG and Rajan, R. Textbook of Medical Biochemistry (2nd edition). Orient Longman.
  • Methods in Plant Biochemistry and Molecular Biology. 1997. Dashek, WV (Ed.). CRC Press.
  • Wilson and Walker. Practical Biochemistry: Principles and Techniques. Cambridge University Press. U.K.

 

 

References: 

 

e-Resourses:

 

Academic Year: 
2023-2024
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