Practical

Practical Exercises based on BOT 121- 124

BOT 121: Cell & Molecular Biology

• Isolation of RNA from Yeast
• Restriction Digestion
• Agarose – Gel Electrophoresis
• SDS- PAGE
• Isolation of DNA from plant material

BOT 122: Cytology & Cytogenetics

• Study of general cytological techniques
• Study of various stages of mitosis in Onion root tips
• Study of various stages of meiosis in Onion flower bud
• Calculation of mitotic index
• To study B chromosomes in plants
• Study of pollen viability
• To measure different types of plant cells using Stage micrometer and Ocular micrometer
• Induction of polyploidy in plants by treating shoot apical meristem with colchicines solution

BOT 123: Hierarchy of Plant Kingdom

• Preparation of report on Indian contributors in the field of algal, fungal, bryophytic, gymnospermic and angiospermic paleobotany
• Microscopic examination of stained cell preparation of bacteria by various staining methods
• Study of role of bacteria in transformation of milk to milk products and in carbohydrate fermentation
• Determination of Biological Oxygen Demand in pure water and water with algal growth to various degrees
• Estimation of protein content in Spirulina, the Single Cell Protein
• Study of mushroom cultivation
• Study of moisture retaining capacity of peat moss
• Study of economic importance of Gymnosperms
• Study of different types of lichen thalli and their role in succession

BOT 124: Microbiology, Fungi & Plant Pathology

• Cleaning of glassware
• To study different methods of sterilization and disinfection by physical agents.
• Instrumentation
• To study various types of culture media
• To determine the growth curve in bacteria (E.coli)
• To study the bacteria by Gram staining
• To become acquainted with Kirby-Baur procedure
• Wine production by fermentative activities of Yeast cells
• Isolation of micro organisms from the mixed culture by spread plate methods
• Isolation of micro organism by pour plate method
• To study ecological relationship between micro organisms of soil by Wino Gradsky Column
• To study the presence of faecal contamination in the given water sample
• Survey of dermatological diseases caused by Fungi
• Inventry preparations of antibiotics procured from Fungi
• Morphological study of representative members of Fungi- Pilobolus, Chaetomium, Morchella, Melampsora, Polyporus, Dreschlera, Phoma, Peziza, Puccinia
• Symptomatology, identification, causal organism of the following diseases: White rust, bunt, smut, green ear, smut and ergot of Bajra, rust and smut of wheat, paddy blast, cotton wilt, downy mildew and powdery mildew of grapes, tundu, tobacco mosaic virus, little leaf of brinjal, root knot of vegetables
• Report on eminent contributors in Mycology, Plant pathology and important related institutes