Coming soon
Practical
Course Objectives
This course will enable the students to-
- To develop skills related to plant tissue culture and applied botany
Course Outcomes (COs):
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Course |
Course Outcomes |
Learning and teaching strategies |
Assessment Strategies |
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Paper Code |
Paper Title |
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BOT 603 |
Practical |
CO95: To have hands on training on different techniques of plant propagation such as cutting, grafting and layering. CO96: To learn the essentials of tissue culture for plant propagation. CO97: To have practical knowledge of various garden operations. CO98:To have an insight into the role of plants in cosmetic industry, medicine, environmental cleanup and energy sector
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Substantial laboratory-based practical component and experiments
Technology-enabled learning
Field-based learning Project-based learning
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Practical assignments and laboratory reports Observation of practical skills Individual and group project reports Viva voce interviews are majorly adopted assessment methods |
- Study of laboratory requirements and general techniques of biotechnology.
- Principle and application of the following- Laminar Air Flow, Autoclave and Ultracentrifugation.
- Preparation of different types of tissue culture medias and solutions of nutrients and growth regulators
- Demonstration of inoculation technique, asceptic transfer of explant, optimizing the conditions for direct/ indirect differentiation in different explants to study totipotency, cytodifferentiation and organogenesis.
- Germination of pollen of Catharanthus roseus in 10 % sucrose solution.
- Demonstration of the technique of protoplast isolation and somatic embryogenesis in Daucus carota.
- Demonstration of the technique of anther culture in Datura.
- Demonstration of the technique of gel electrophoresis of bacterial DNA.
- Demonstration of the phenomenon of action of Restriction Endonucleases.• Field study of various plant forms- climbers, trees, shrubs, orchids, ferns and palms.
- Experimental study of the technique of vegetative propagation-cutting, budding, grafting and air layering etc.
- To study propagation by means of bulbs, corms, tubers, rhizome etc.
- To study different styles of garden design.
- Field visit to understand different patterns of landscape design.
- Preparation of record on local biodiversity.
- Bendre and Kumar Vol.I Rastogi Publication
- Home Gardening-Pratibha Trivedi
- Applied Botany-P.C.Trivedi
- College Botany Practical Vol. I and II - Santia S.C.
- Gardening in India -Lancaster’s Percy
