Plant Physiology and Biochemistry II

Paper Code: 
BOT 224
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

To study the various biochemical pathways in plants and to study the structure of various biomolecules.

12.00
Unit I: 

Carbohydrates: Classification, catabolism and anabolism of carbohydrates, Chemistry, conformation and functions of monosaccharides, disaccharides, oligosaccharides, polysaccharides and glycoproteins including starch, cellulose and pectins.

                                                                                                  (12 Hours)

12.00
Unit II: 

Proteins: Structure of protein: primary , secondary ,tertiary, quaternary, Ramachandran plot, techniques of protein purification, ion exchange chromatography, size exclusion chromatography, protein folding, chemical and enzymatic hydrolysis of protein to peptides, composition and sequence analysis of proteins: N and C terminal analysis.

12.00
Unit III: 

Fat metabolism: Introduction, classes of lipids, lipid structure, metabolism of lipids, and function of derived lipids: phospholipids, sphingolipids, cholesterol, plasmalogens and glycerolipids.

Vitamins: Biochemistry and function of thiamine, riboflavin, nicotinic acid, pantothenic acid, pyridoxine, biotin, folic acid, vitamin B12, ascorbic acid, vitamin A , vitamin D, vitamin E and  vitamin K, clinical aspect of vitamin deficiency.

12.00
Unit IV: 

Enzymes: Introduction to enzyme, classification, regulation of enzyme activity, mechanism of action, enzyme kinetics, Michaelis-Menten equation, coenzymes, isozymes, ribozymes and abzymes.                                            

12.00
Unit V: 

Secondary metabolites: Biosynthesis and functions of secondary metabolites with special reference to alkaloids, tannins, lignins, sapogenins, coumarins, cardiac glycosides and anthocyanins.

                                                                                                                         

References: 
  1. Buchanan, BB; Gruissem, W and Jones, RL. 2000. Biochemistry and Molecular Biology of Plants. American Society of Plant Physiologists. Maryland, USA
  2. Bugg, TDH. 2004. Introduction of Enzyme and Co-enzyme chemistry (2nd edition). Blackwell Publishing Ltd., UK.
  3. Gurr, MI; Harwood, JL and Frayn, KN. Lipid Biochemistry: An Introduction (5th edition). Blackwell Science.
  4. Hames, BD and Hooper, NM. 2003. Biochemistry: Instant Notes (2nd edition). Bios Scientific Publishers Ltd.    
  5. Lea, PJ and Leegood, RC. 1999. Plant Biochemistry and Molecular Biology. John
  6. Wiley & Sons, New York.
  7. Lesk, AM. 2001. Introduction to Protein Architechture. Oxford University Press, UK
  8. Lydyard, PM; Whelan, A and Fanger, MW. 2003. Instant Notes in Immunology. Hames, BD (ed.). Viva Books Pvt. Ltd., New Delhi
  9. Mathews, CK; van Holde, KE and Ahern, KG. 2003. Biochemistry. Pearson Education Pvt. Ltd., New Delhi.
  10. Mohr, H and Schopfer, P. 1995. Plant Physiology. Springer- Verlag, Berlin, Germany.
  11. Montgomery, R; Conway, TW and Spector, AA. 1990. Biochemistry: A Case Oriented Approach (5th edition). The CV Mosby Co., Torento.
  12. Principles and Techniques of Biochemistry and Molecular Biology (6th edition). Wilson, K and Walker, J (eds.). Cambridge University Press
  13. Rawn, DJ. Biochemistry. Panima Publishing Co., New Delhi.
  14. Sands. 1995. Problems in Plant Physiology. John Murray, London.
  15. Srivastava, HN. 2006. Pradeep’s Botany Vol. V. Pradeep Publications, Jalandhar
  16. Thimmaiah, SR. 2004. Standard Methods of Biochemical Analysis. Kalyani Publishers.
  17. Trehan, K. 1990. Biochemistry (2 nd edition.). New Age International (P) Ltd., Publishers, New Delhi.
  18. Voet, D and Voet, J. 1995. Biochemistry (2nd edition). John Wiley & Sons Inc.. New York.
  19. West, ES; Todd, WR; Mason, HS and Bruggen, JTV. 1974. Textbook of Biochemistry (4th edition). Oxford & IBH Publishing Co. Pvt. Ltd., New Delhi.
  20. Zubay, GL; Parson, WW and Vance, DE. Principles of Biochemistry.

Laboratory Readings-

  1. Ramakrishnan, S; Prasannan, KG and Rajan, R. Textbook of Medical Biochemistry (2nd edition). Orient Longman.
  2. Plummer. An Introduction to Practical Biochemistry.
  3. Singh, S. Practical Manual of Biochemistry.
Academic Year: 
2018-2019
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