Plant Biotechnology and Bioinformatics

Paper Code: 
BOT324
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course Objectives:

This course will enable the students to –

  1. To study the various tools and techniques used to improve the plant productivity and to introduce the basic aspects of bioinformatics.
  2. To impart knowledge and practical skills to use biotechnological tools in crop improvement.
  3. To impart knowledge about the methods of molecular separation and characterization
  4. To expose the practical nuances of biotechnology, computing sciences and information tools by way of lab visits.

Course Outcomes (COs):

     Course

Course Outcome

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BOT 324

 

Plant Biotechnology and Bioinformatics

CO72:  Knowledge of various cyber issues concerning IPR

CO73:  Demonstrate knowledge and understanding of the appropriate procedures for obtaining Intellectual Property Protection

CO74:  Understand the concept of micro propagation through somatic embryogenesis and organogenesis in plants

CO75:  Demonstrate the core concepts of Bioinformatics, including computational biology, database design and implementation and probability.

CO76:  Learn the specific and non-specific methods of gene transfer

CO77:  Learn the technique of Recombinant DNA technology and its applications in human welfare

CO78:  Understand basic laboratory setup, handling of explant tissue, nutrient medium and establishing the culture and incubation of cultures.

CO79:  Deals with fundamentals of bioinformatics tools, computational biology and statistical methods utmost necessary for contemporary research

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question preparation

 

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects
 

 

12.00
Unit I: 
Plant tissue culture

General introduction, history and scope of plant tissue culture, comparision of different plant tissue culture media, concept of totipotency, organogenesis, somatic embryogenesis (direct & indirect), callus culture and suspension culture, somaclonal variation, hardening and acclimatization of plants. 

 Protoplast culture: Isolation and culture of protoplast, somatic hybridization, hybrid selection and regeneration, concept of hybrid and cybrid, achievements and limitations of protoplast culture

12.00
Unit II: 
Transgenic plants

Concept and history of transgenesis in plants, principles and techniques of gene cloning: direct DNA transfer to plant cells, Agrobacterium mediated transformation: Ti plasmid, process of T- DNA transfer and integration, vectors, promoter, terminator, marker and reporter genes, ethical and ecological issues of transgenesis, terminator technology (GURT)

 

12.00
Unit III: 
Intellectual Property Rights

Introduction of IPR, Forms of IPRs, Distinction among various IPRs; Introduction of Paris convention, WIPO, WTO and TRIPS, Indian IP system; Salient features of Patent Act, patentability requirement, prior-art search, Documents  requirement for filing an application for patent, patent publication, technology transfer, Infringements and remedies; Budapest treaty; Protection of plant variety and farmers rights Act, Convention of Biological Diversity, bio-patents, traditional knowledge, case studies – haldi, neem, basmati, GM crops and IPRs, Geographical indicators.

 

12.00
Unit IV: 
Genetic engineering

Molecular tools and their applications: Restriction enzymes, C DNA and genomic library, DNA sequencing, polymerase chain reaction, DNA fingerprinting.                                                                                      

 

12.00
Unit V: 
Bioinformatics

Gene bank sequence database, structure database, sequence analysis, information retrieval from biological database, NCBI data model, sequence alignment and database searching and practical aspects of multiple sequence alignment

Essential Readings: 
  • Bhojwani, SS. 1990. Plant Tissue Culture: Applications and Limitations.
  • Bhojwani, SS and Razdan, MK. 1996. Plant Tissue Culture: Theory and Practice (a revised edition). Elsevier Science Publishers. New York, USA.
  • Brown, TA.1999.Genomes. John Wiley & Sons (Asis) Pvt. Ltd., Singapore.
  • Callow, JA; Ford-Lloyd, BV and Newbury, HJ. 1997. Biotechnology and Plant Genetic Resources: Conservation and Use. CAB International, Oxon, UK.
  • Chawla, HS. 2002. Introduction to Plant Biotechnology. Oxford & IBH Publishing Co. Pvt. Ltd.
  • Chrispeels, MJ and Sadava, DE. 1994. Plants, Genes and Agriculture. Jones and Barlett Publishers. Boston, USA.
  • Collins, HA and Edwards, S. 1998. Plant Cell Culture. Bios Scientific Publishers. Oxford, UK.
  • Gustafson, JP. 2000.Genomes. Kluwer Academic Plenum Publishers. New York.
  • Jolles, O and Jomvall, H.(eds.) .2000. Proteomics in Functional Genomics. Birkhauser Verlag, Basel, Switzerland.
  • Kartha, KK. 1985. Cryopreservation of Plant Cells and Organs.
  • Primrose, SB. 1995. Principles of Genome Analysis. Blackwell ScienceLtd. Oxford, UK.
  • Edwards and Sue. 1998. Plant Cell Culture. Bios Scientific Publishers, London.
  • Kumar, U. 2001. Methods in Plant Tissue Culture. Agrobios, Jodhpur.
  • Ignacimuthu, S. 2001. Plant Biotechnology. Oxford & IBH Publishing Co. Pvt. Ltd.
  • Murphy and Terence, M. 1998. Plant Biology. Wadsworth Publishing Co., California.
  • Narayanswamy. 1994. Plant Cell and Tissue Culture. Tata McGraw Hill , New Delhi.
  • Shantharam, S and Montgomery, JF. 1999. Biotechnology, Biosafety and Biodiversity. Oxford & IBH Publishing Co. Pvt. Ltd., New Delhi
  • Singh and Rita. 2004. Plant Biotechnology. Global Vision Publishing House.

 

 

Academic Year: 
2019-2020
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