Plant Biotechnology

Paper Code: 
BOT 601
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 

To study the tools and techniques of plant biotechnology.

 

 

Unit I: 
Genetic engineering I

Restriction endonucleases, cutting and joining of DNA molecules, cloning vectors: plasmids, cosmids, bacteriophages, phagemids and YAC. Genomic and cDNA library.

 

 

Unit II: 
Genetic engineering II

Gene transfer by Agrobacterium, transgenic crops- Bt cotton, Golden rice, Flavr savr tomato, edible vaccines, Intellectual Property Rights (IPR).

 

Unit III: 
Tools and Techniques I

Gel electrophoresis, Agarose gel electrophoresis, Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS PAGE), High Performance Liquid Chromatography (HPLC), Polymerase Chain Reaction (PCR), EnzymeLlinked Immunosorbent Assay (ELISA)

Unit IV: 
Tools and Techniques II

Flow cytometry, ultracentrifugation, FISH (Fluorescent in situ hybridization), GISH (Genomic in situ hybridization), SEM (Scanning Eelectron   Microscopy), TEM (Transmission   Electron Microscopy), mass spectrometry.

                        

Unit V: 
Plant tissue culture

Basic aspects of plant tissue culture, Cellular totipotency, micropropagation and organogenesis, somatic embryogeneis, artificial seeds, Isolation and culture of protoplast, somatic hybridization and cybridization, anther culture.

 

 

Essential Readings: 
  • Bhojwani, SS and Razdan, MK. 1996. Plant Tissue Culture: Theory and Practice (a revised edition). Elsevier Science Publishers. New York, USA.
  • Brown, TA.1999.Genomes. John Wiley & Sons (Asis) Pvt. Ltd., Singapore.
  • Callow, JA; Ford-Lloyd, BV and Newbury, HJ. 1997. Biotechnology and Plant Genetic Resources: Conservation and Use. CAB International, Oxon, UK.
  • Chawla, HS. 2002. Introduction to Plant Biotechnology. Oxford & IBH Publishing Co. Pvt. Ltd.
  • Primrose, SB. 1995. Principles of Genome Analysis. Blackwell Science Ltd. Oxford, UK.

 

 

 

References: 
  • Chrispeels, MJ and Sadava, DE. 1994. Plants, Genes and Agriculture. Jones and Barlett Publishers. Boston, USA.
  • Collins, HA and Edwards, S. 1998. Plant Cell Culture. Bios Scientific Publishers. Oxford, UK.
  • Gustafson, JP. 2000. Genomes. Kluwer Academic Plenum Publishers. New York.
  • Jolles, O and Jomvall, H(eds.) .2000. Proteomics in Functional Genomics. Birkhauser Verlag, Basel, Switzerland.
  • Kartha, KK. 1985. Cryopreservation of Plant Cells and Organs.
  • Edwards and Sue. 1998. Plant Cell Culture. Bios Scientific Publishers, London.
  • Ignacimuthu, S. 2001. Plant Biotechnology. Oxford & IBH Publishing Co. Pvt. Ltd.
  • Murphy and Terence, M. 1998. Plant Biology. Wadsworth Publishing Co., California.
  • Narayanswamy. 1994. Plant Cell and Tissue Culture. Tata McGraw Hill , New Delhi.
  • Shantharam, S and Montgomery, JF. 1999. Biotechnology, Biosafety and Biodiversity. Oxford & IBH Publishing Co. Pvt. Ltd., New Delhi
  • Singh and Rita. 2004. Plant Biotechnology. Global Vision Publishing House.
  • Vasil, IK and Thorpe, TA. 1994. Plant Cell and Tissue Culture. Kluwer Academic Publishers, The Netherlands

 

 

 

Academic Year: 
2019-2020
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