PLANT BIOCHEMISTRY-P

Paper Code: 
25DBOT502A
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students: To develop skills related to qualitative and quantitative estimations of biomolecules

 

Course Outcomes: 

Course

Learning Outcome

(at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course title

25DBOT

502A

PLANT BIOCHEMISTRY-P

 

CO47: Develop holistic competencies viv-a-vis verbal and written communication skills, which will promote curiosity, innovation, and a passion for research including critical thinking

CO48: Analyze and separate biomolecules like proteins, carbohydrate pigments and develop an understanding of enzyme activity

CO49: Design experiments to determine the composition of complex molecules like primary and secondary metabolites

CO50: Contribute effectively in course-specific interaction.

 

Approach in teaching: Reading assignments, Demonstration, Team teaching, Substantial laboratory-based practical component and experiments

Technology-enabled learning

 

Learning activities for the student:

Effective questions, Stimulation, Seminar presentation.

Practical assignments and laboratory reports

Observation of practical skills

Individual and group project reports

Viva voce interviews are majorly adopted assessment methods
 

 

 

  CONTENTS:

  • Estimation of the following- glucose, starch, proteins, fats, tannins, ascorbic acid and anthocyanins.
  • Demonstration of enzyme activity- peroxidase, and catalase in plant tissue samples and measurement of their activities.
  • Quantitative Estimation of protein content by Folin – Lowry method
  • Quantitative Estimation of protein content by Biuret Reagent method.
  • Anatomical study of root nodule depicting symbiotic relationship between nitrogen fixing bacteria and roots of leguminous plants.
  • Separation of plant pigments & dyes using chromatographic techniques (paper, TLC)
  • Separation of amino acids using chromatographic techniques (paper, TLC).
  • Estimation of secondary metabolites as prescribed in the syllabus using Soxhlet apparatus.

 

Scheme of Evaluation

Continuous Assessment

 

Semester End Examination

 

Component

 

Marks

 

 

Component

 

Marks

 

Test

 

10

Phytochemical analysis of primary and secondary metabolites.

 

20

Attendance

05

Estimation of protein content, enzyme activity and separation of plant pigments and amino acids.

 

20

 

Spotting

 

10

Viva voce

05

 

Viva voce

10

Practical Record

 

10

Practical Record

 

10

 

Total

 

30

 

Total

 

70
 

 

 

Essential Readings: 
  • Dashek, W. V. (2018). Methods in plant biochemistry and molecular biology. CRC Press.
  • Wilson, K., Wilson, K., & Walker, J. (2005). Practical Biochemistry: Principles and Techniques. U.K: Press Syndicate of the University of Cambridge.
  • Bendre, A. (2020). Practical botany: Plant physiology, biochemistry, biotechnology, ecology and plant utilization. Meerut, India: Rastogi Publications.
  • Santra, S. C. (2020). Practical Botany. La Vergne: New Central Book Agency.
  • Harborne, J. B. (2012). Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis, 3E. India: Springer.
  • Damodaran, G. K., K, G. D. (2016). Practical Biochemistry. India: Jaypee Brothers Medical Publishers Pvt. Lited.
  • Soundravally, A., Dhiman, P., R, (2019). Biochemistry Practical Manual E-Book. books.google.co.in › books
  • Wood, E. J. (2012). Practical Biochemistry for Colleges. United Kingdom: Elsevier Science.
  •  Sawhney, S. K. (2005). Introductory Practical Biochemistry. Unitedju Kingdom: Alpha Science International.
  • Ramakrishnan, S; Prasannan, KG and Rajan, R. Textbook of Medical Biochemistry (2nd edition). Orient Longman.
  • Methods in Plant Biochemistry and Molecular Biology. 1997. Dashek, WV (Ed.). CRC Press.
  • Wilson and Walker. Practical Biochemistry: Principles and Techniques. Cambridge University Press. U.K.

 

References: 

e-Resourses:

 

 

Academic Year: 
2025-2026
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