Plant Biochemistry (Discipline Specific Elective)

Paper Code: 
DBOT 501A
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to-

  1. To study the various biochemical pathways in plants and the structure of various biomolecules.
  2. To study the critical role of secondary metabolites in plant physiology and their defence mechanism

 

Course Outcomes (COs):

Course Outcomes

Learning and teaching strategies

Assessment Strategies

 
 

On completion of this course, the students will be able to;

CO37:  Develop an understanding of recent developments in biosynthesis of different classes of carbohydrates, proteins and fats and their functions.

CO38:  Understand the biochemistry of nitrogen fixation and apply its knowledge in agriculture and related disciplines.

CO39:  Understand the structure and function of fatty acids, their biosynthesis and oxidation

CO40:  Describe the mechanisms of Enzyme action, activation, catalysis and their properties and factors affecting enzyme activity.

CO41:  Understand the structure and function of secondary metabolites and interpret their commercial applications.

Class Hours

Seminars

Tutorials

Group discussions and Workshops

Question Framing

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

 
 

 

12.00
Unit I: 
Carbohydrates

Carbohydrates: Classification, structure and functions of monosaccharides, disaccharides, polysaccharides and glycoproteins including starch, cellulose and pectins.

          

12.00
Unit II: 
Proteins

Proteins: Structure of proteins: Primary, Secondary, Tertiary, Quaternary; Simple and conjugated proteins, Synthesis of amino acids by reductive amination and transamination, Zwitter ion 

Nitrogen metabolism: N2 cycle, mechanism, biology and chemistry of nitrogen fixation, root nodulation, Nod factor, asymbiotic N2 fixation, GS-GOGAT system.

 

 

12.00
Unit III: 
Fat Metabolism

Fat metabolism: Saturated and Unsaturated fatty acids, Synthesis of long chain fatty acids; Structure, functions and biosynthesis of lipids, α-oxidation, β-oxidation

 

12.00
Unit IV: 
Enzymes

Enzymes: Structure and properties, concept of holoenzyme, apoenzyme, coenzyme and cofactors, regulation of enzyme activity, mechanism of action, Factors affecting enzyme activity, enzyme kinetics, Michaelis-Menten equation.

 

12.00
Unit V: 
Secondary Metabolism

Secondary metabolites: Structure and functions of secondary metabolites: Alkaloids, tannins, terpenes, sterols and anthocyanins.

 

Essential Readings: 
  • Stryer L., Berg J., Tymoczko J. (2015); Biochemistry (Ninth Edition); W. H. Freeman publications. Pages: 1296
  • Buchanan, B.B.; Gruissem, W. and Jones, R.L. (2000). Biochemistry and Molecular Biology of Plants. American Society of Plant Physiologists. Maryland, USA
  • Srivastava, H.N. (2006). Pradeep’s Botany Vol. V. Pradeep Publications, Jalandhar
  • Voet, D. and Voet, J. (2010). Biochemistry (2nd edition). John Wiley & Sons Inc. New York.
  • Mohr, H. and Schopfer, P. (1995). Plant Physiology. Springer- Verlag, Berlin, Germany.
  • Lehninger. (2017). Principles of Biochemistry. Wadsworth Publishing Co. California, USA.
  • Hegde, P.H. (2005). Text Book of Biochemistry. Mohit Publications, New Delhi.
  • Lea, P.J. and Leegood, R.C. (1999). Plant Biochemistry and Molecular Biology. John Wiley & Sons, New York.
  • Lesk, A.M. (2010). Introduction to Protein Architechture. Oxford University Press, UK
  • Mathews, C.K.; Van Holde, K.E. and Ahern, K.G. (2003). Biochemistry. Pearson Education Pvt. Ltd., New Delhi.
  • Montgomery, R.; Conway, T.W. and Spector, A.A. (1996). Biochemistry: A Case Oriented Approach (5th edition). The CV Mosby Co., Toronto.

 

References: 
  • Principles and Techniques of Biochemistry and Molecular Biology (6th edition). (2010); Wilson, K and Walker, J (Eds.). Cambridge University Press
  • Sands. (1995). Problems in Plant Physiology. John Murray, London.
  • Thimmaiah, S.R. (2004). Standard Methods of Biochemical Analysis. Kalyani Publishers.
  • Trehan, K. (1990). Biochemistry (2 nd edition.). New Age International (P) Ltd., Publishers, New Delhi.
  • West, E.S.; Todd, W.R.; Mason, H.S. and Bruggen, J.T.V. (1974). Textbook of Biochemistry (4th edition). Oxford & IBH Publishing Co. Pvt. Ltd., New Delhi.
  • Zubay, G.L.; Parson, W.W. and Vance, D.E. (1995). Principles of Biochemistry.
  • Jain J. L.; Jain S.; Jain N. (2016). Fundamentals of Biochemistry. S.Chand Publisher, New Delhi.

 

e-Resourses:

 

Journals:

  • Annual Review of Biochemistry (Annual Reviews (US))
  • International Journals of Biochemistry and Cell Biology (Elsevier Publishing Company)
  • Journals of Biochemistry (Oxford University Press)
  • Journals of Plant Biochemistry and Biotechnology (Springer India)

 

 

Academic Year: 
2023-2024
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