PLANT BIOCHEMISTRY

Paper Code: 
24DBOT501A
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to-

  1. To study the various biochemical pathways in plants and the structure of various biomolecules.
  2. To study the critical role of secondary metabolites in plant physiology and their defense mechanism

 

Course Outcomes: 

Course

Learning Outcome

(at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course title

24DBOT501A

PLANT BIOCHEMISTRY

 

CO41: Appraises knowledge about the classification, structure, and functions of carbohydrates with elaborate knowledge about polysaccharides and glycoproteins.

CO42: Develop an understanding of structure of proteins and build a knowledge of reductive amination synthesis of amino acids

CO43: Discuss fat metabolism, synthesis of fatty acids and determine their functions.

CO44: analyse and assess the mechanism of enzyme action activation, catalysis and their properties

CO45: Maximize knowledge about secondary metabolite synthesis and propose their commercial application

CO46: Contribute effectively in course-specific interaction.

Approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

Learning activities for the student:

Self-learning assignment, Effective questions, Stimulation, Seminar presentation

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects
 

 

12.00
Unit I: 
Carbohydrates

Classification, structure and functions of monosaccharides, disaccharides, polysaccharides and glycoproteins including starch, cellulose and pectins.

                    

12.00
Unit II: 
Proteins

Structure of proteins: Primary, Secondary, Tertiary, Quaternary; Simple and conjugated proteins, Synthesis of amino acids by reductive amination and transamination, Zwitter ion 

Nitrogen metabolism: N2 cycle, mechanism, biology and chemistry of nitrogen fixation, root nodulation, Nod factor, asymbiotic N2 fixation, GS-GOGAT system.

 

 

12.00
Unit III: 
Fat Metabolism

Saturated and Unsaturated fatty acids, Synthesis of long chain fatty acids; Structure, functions and biosynthesis of lipids, α-oxidation, β-oxidation

 

12.00
Unit IV: 
Enzymes

Structure and properties, concept of holoenzyme, apoenzyme, coenzyme and cofactors, regulation of enzyme activity, mechanism of action, Factors affecting enzyme activity, enzyme kinetics, Michaelis-Menten equation.

 

12.00
Unit V: 
Secondary Metabolites

Structure and functions of secondary metabolites: Alkaloids, tannins, terpenes, sterols and anthocyanins.

                              

 

Essential Readings: 
  • Stryer L., Berg J., Tymoczko J. (2015); Biochemistry (Ninth Edition); W. H. Freeman publications. Pages: 1296
  • Buchanan, B.B.; Gruissem, W. and Jones, R.L. (2000). Biochemistry and Molecular Biology of Plants. American Society of Plant Physiologists. Maryland, USA
  • Srivastava, H.N. (2006). Pradeep’s Botany Vol. V. Pradeep Publications, Jalandhar
  • Voet, D. and Voet, J. (2010). Biochemistry (2nd edition). John Wiley & Sons Inc. New York.
  • Mohr, H. and Schopfer, P. (1995). Plant Physiology. Springer- Verlag, Berlin, Germany.
  • Lehninger. (2017). Principles of Biochemistry. Wadsworth Publishing Co. California, USA.
  • Hegde, P.H. (2005). Text Book of Biochemistry. Mohit Publications, New Delhi.
  • Lea, P.J. and Leegood, R.C. (1999). Plant Biochemistry and Molecular Biology. John Wiley & Sons, New York.
  • Lesk, A.M. (2010). Introduction to Protein Architechture. Oxford University Press, UK
  • Mathews, C.K.; Van Holde, K.E. and Ahern, K.G. (2003). Biochemistry. Pearson Education Pvt. Ltd., New Delhi.
  • Montgomery, R.; Conway, T.W. and Spector, A.A. (1996). Biochemistry: A Case Oriented Approach (5th edition). The CV Mosby Co., Toronto.

 

References: 
  • Principles and Techniques of Biochemistry and Molecular Biology (6th edition). (2010); Wilson, K and Walker, J (Eds.). Cambridge University Press
  • Sands. (1995). Problems in Plant Physiology. John Murray, London.
  • Thimmaiah, S.R. (2004). Standard Methods of Biochemical Analysis. Kalyani Publishers.
  • Trehan, K. (1990). Biochemistry (2 nd edition.). New Age International (P) Ltd., Publishers, New Delhi.
  • West, E.S.; Todd, W.R.; Mason, H.S. and Bruggen, J.T.V. (1974). Textbook of Biochemistry (4th edition). Oxford & IBH Publishing Co. Pvt. Ltd., New Delhi.
  • Zubay, G.L.; Parson, W.W. and Vance, D.E. (1995). Principles of Biochemistry.
  • Jain J. L.; Jain S.; Jain N. (2016). Fundamentals of Biochemistry. S.Chand Publisher, New Delhi.

 

e-Resourses:

 

Journals:

  • Annual Review of Biochemistry (Annual Reviews (US))
  • International Journals of Biochemistry and Cell Biology (Elsevier Publishing Company)
  • Journals of Biochemistry (Oxford University Press)
  • Journals of Plant Biochemistry and Biotechnology (Springer India)

 

 

Academic Year: 
2024-2025
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