Plant Biochemistry

Paper Code: 
BOT 502
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 

Course Objectives:

This course will enable the students to-

  1. To study the various biochemical pathways in plants and the structure of various biomolecules.
  2. To study the critical role of secondary metabolites in plant physiology and their defence mechanism

Course Outcomes (COs):

Course

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BOT 502

 

Plant Biochemistry

On completion of this course, the students will be able to;

CO73:  Illustrate and explain the structure and properties of Macromolecules

CO74:  Understand the biosynthesis of various secondary metabolites.

CO75:  Describe the mechanisms of Enzyme action, activation, catalysis and their properties.

CO76:  Classify various pathways for the biosynthesis of tannins, phenols and nitrogenous compounds.

CO77:  Develop an understanding of recent developments of latest methods of biosynthesis of carbohydrates, proteins and fats.

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question Framing

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

 

 

9.00
Unit I: 
Carbohydrates

Carbohydrates: Classification, structure and functions of monosaccharides, disaccharides, polysaccharides and glycoproteins including starch, cellulose and pectins.

 

9.00
Unit II: 
Proteins

Proteins: Structure of proteins: Primary, Secondary, Tertiary, Quaternary; Simple and conjugated proteins, Synthesis of amino acids by reductive amination and transamination, Zwitter ion 

Nitrogen metabolism: N2 cycle, mechanism, biology and chemistry of nitrogen fixation, root nodulation, Nod factor, asymbiotic N2 fixation, GS-GOGAT system.

 

 

9.00
Unit III: 
Fat metabolism

Fat metabolism: Saturated and Unsaturated fatty acids, Synthesis of long chain fatty acids; Structure, functions and biosynthesis of lipids, α-oxidation, β-oxidation

 

9.00
Unit IV: 
Enzymes

Enzymes: Structure and properties, concept of holoenzyme, apoenzyme, coenzyme and cofactors, regulation of enzyme activity, mechanism of action, Factors affecting enzyme activity, enzyme kinetics, Michaelis-Menten equation.

 

9.00
Unit V: 
Secondary metabolites

Secondary metabolites: Structure and functions of secondary metabolites: Alkaloids and tannins, cardiac glycosides and anthocyanins.

                                     

 

 

 

Essential Readings: 
  • Stryer L., Berg J., Tymoczko J. (2015); Biochemistry (Ninth Edition); W. H. Freeman publications. Pages: 1296
  • Buchanan, B.B.; Gruissem, W. and Jones, R.L. (2000). Biochemistry and Molecular Biology of Plants. American Society of Plant Physiologists. Maryland, USA
  • Srivastava, H.N. (2006). Pradeep’s Botany Vol. V. Pradeep Publications, Jalandhar
  • Voet, D. and Voet, J. (2010). Biochemistry (2nd edition). John Wiley & Sons Inc. New York.
  • Mohr, H. and Schopfer, P. (1995). Plant Physiology. Springer- Verlag, Berlin, Germany.
  • Lehninger. (2017). Principles of Biochemistry. Wadsworth Publishing Co. California, USA.
  • Hegde, P.H. (2005). Text Book of Biochemistry. Mohit Publications, New Delhi.
  • Lea, P.J. and Leegood, R.C. (1999). Plant Biochemistry and Molecular Biology. John Wiley & Sons, New York.
  • Lesk, A.M. (2010). Introduction to Protein Architechture. Oxford University Press, UK
  • Mathews, C.K.; Van Holde, K.E. and Ahern, K.G. (2003). Biochemistry. Pearson Education Pvt. Ltd., New Delhi.
  • Montgomery, R.; Conway, T.W. and Spector, A.A. (1996). Biochemistry: A Case Oriented Approach (5th edition). The CV Mosby Co., Toronto.

 

References: 

e-Resourses:

Journals:

  • Annual Review of Biochemistry (Annual Reviews (US))
  • International Journals of Biochemistry and Cell Biology (Elsevier Publishing Company)
  • Journals of Biochemistry (Oxford University Press)
  • Journals of Plant Biochemistry and Biotechnology (Springer India)

 

Academic Year: