Plant Biochemistry

Paper Code: 
BOT502
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 
 

Course Objectives:

This course will enable the students to-

  1. To study the various biochemical pathways in plants and the structure of various biomolecules.
  2. To provide an overview of the plant biochemical and physiological processes that could be exploited both to reduce the environmental impact of modern agricultural practices and to develop strategies and tools to increase crop yield and quality

Course Outcomes (COs):

Course

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BOT 502

 

Plant Biochemistry

CO73:  Study the structure and properties of Macromolecules

CO74:  Understand the biosynthesis of various secondary metabolites.

CO75:  Learn the properties, Enzyme catalysis and activation energy and mechanism of enzyme action.

CO76:  Understand the biosynthesis of tannins, phenols and nitrogenous compounds.

 

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question Framing

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects
 

 

9.00
Unit I: 
Carbohydrates:

Classification, structure and functions of monosaccharides, disaccharides, polysaccharides and glycoproteins including starch, cellulose and pectins.

 

9.00
Unit II: 
Proteins and Nitrogen metabolism

Proteins: Structure of proteins: Primary, Secondary, Tertiary, Quaternary; Simple and conjugated proteins, Synthesis of amino acids by reductive amination and transamination, Zwitter ion

Nitrogen metabolism: N2 cycle, mechanism, biology and chemistry of nitrogen fixation, root nodulation, Nod factor, asymbiotic N2 fixation, GS-GOGAT system.

 

9.00
Unit III: 
Fat metabolism:

Saturated and Unsaturated fatty acids, Synthesis of long chain fatty acids; Structure, functions and biosynthesis of lipids, α-oxidation, β-oxidation

 

9.00
Unit IV: 
Enzymes:

Structure and properties, concept of holoenzyme, apoenzyme, coenzyme and cofactors, regulation of enzyme activity, mechanism of action, Factors affecting enzyme activity, enzyme kinetics, Michaelis-Menten equation.

 

9.00
Unit V: 
Secondary metabolites:

Structure and functions of secondary metabolites: Alkaloids and tannins, cardiac glycosides and anthocyanins.

 

Essential Readings: 
  • Buchanan, BB; Gruissem, W and Jones, RL. 2000. Biochemistry and Molecular Biology of Plants. American Society of Plant Physiologists. Maryland, USA
  • Srivastava, HN. 2006. Pradeep’s Botany Vol. V. Pradeep Publications, Jalandhar
  • Voet, D and Voet, J. 1995. Biochemistry (2nd edition). John Wiley & Sons Inc.. New York.
  • Mohr, H and Schopfer, P. 1995. Plant Physiology. Springer- Verlag, Berlin, Germany.
  • Lehninger.2000. Principles of Biochemistry. Wadsworth Publishing Co. California, USA.
  • J.L. Jain. Fundamentals of Biochemistry. S.Chand and Co., New Delhi.

 

 

References: 
  • Hegde, PH. 2005. Text Book of Biochemistry. Mohit Publications, New Delhi.
  • Lea, P.J. and Leegood, R.C.1999.Plant Biochemistry and Molecular Biology. John Wiley & Sons, New York.
  • Lesk, AM. 2001. Introduction to Protein Architechture. Oxford University Press, UK
  • Mathews, CK; van Holde, KE and Ahern, KG. 2003. Biochemistry. Pearson Education Pvt. Ltd., New Delhi.
  • Montgomery, R; Conway, TW and Spector, AA. 1990. Biochemistry: A Case Oriented Approach (5th edition). The CV Mosby Co., Toronto.
  • Murphy and Terence, M. 1998. Plant Biology. Wadsworth Publishing Co., California.
  • Principles and Techniques of Biochemistry and Molecular Biology (6th edition). Wilson, K and Walker, J (Eds.). Cambridge University Press
  • Rawn, DJ. Biochemistry. Panima Publishing Co., New Delhi.
  • Sands. 1995. Problems in Plant Physiology. John Murray, London.
  • Thimmaiah, SR. 2004. Standard Methods of Biochemical Analysis. Kalyani Publishers.
  • Trehan, K. 1990. Biochemistry (2 nd edition.). New Age International (P) Ltd., Publishers, New Delhi.
  • West, ES; Todd, WR; Mason, HS and Bruggen, JTV. 1974. Textbook of Biochemistry (4th edition). Oxford & IBH Publishing Co. Pvt. Ltd., New Delhi.
  • Zubay, G.L.; Parson, W.W. and Vance, D.E. Principles of Biochemistry.

 

Academic Year: 
2020-2021
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