Paper III: Plant Tissue culture (Advance Paper-I)

Paper Code: 
LSC 143 C
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
12.00
Unit I: 
Plant tissue Culture I:

Introduction to cell and tissue culture; Terms and definitions, Historical developments and milestones in plant tissue culture, special feature and internal organization of plant cells, totipotency, Dedifferentiation & redifferentiation.

 

12.00
Unit II: 
Plant tissue Culture II

Tissue Culture Media : History, Evolution and composition of: White's Media, B5 (Gamborg), MS (Murashige and Skoog), LS (Linsmaier and Skoog), SH (Schank and Hildebrandt) and N6 (Chu et al.) Media.  

 

12.00
Unit III: 
Micro propagation

Axillary bud, shoot tip, meristem culture, Somatic embryogenesis, Cell suspension culture, Protoplast isolation, testing viability of isolated protoplast. Steps in regeneration of protoplast, protoplast fusion: chemical & electrical.

 

12.00
Unit IV: 
Histology of Tissues

Selection of material, chemical fixation - types of fixatives, mechanism of fixation, tissue dehydration - general protocol, rapid dehydration and dehydration using a graded solvent series of ethanol or acetone, infiltrating and embedding tissues, sectioning, mounting and staining, alternate methods of microtomy (cryotome, cryostat and vibratome).

12.00
Unit V: 
Biotransformation

Production of secondary metabolites from plant cell cultures processes for enhancing the production of secondary metabolites, bioreactor system and modules for mass multiplication. Germplasm conservation (Cryopreservation)

 

Essential Readings: 
  • Roberta Smith, Plant Tissue Culture: Techniques and Experiments. 2nd ed.,Academic Press, 2000.
  • Bhojwani, S.S. and Rajdan, Plant Tissue Culture: Theory and Practice. 2004 Roberta Smith, Plant Tissue Culture: Techniques and Experiments, Academic Press; 2nd ed, 2000. Crispeels, M.J. and Sadava, D.E., Plants, Genes and Crop Biotechnology,
  • Jones and Bartlett Publishers (2nd Edition), 2003. Bhowjwani, S.S., Plant Tissue Culture: Application and Limitations. Amsterdam,
  • Elsevier,1990. Charles Cunningham and Andrew J.R. Porter, Recombinant Proteins from Plants: Production and Isolation of Clinically
  • Useful Compounds (Methods in Biotechnology), Humana Press, 1997. Bernard R. Glick and John E. Thompson, Methods in Plant Molecular Biology and Biotechnology, CRC Press, 1993. I. Potrykus and G. Spangenberg,, Gene Transfer to Plants (Springer Lab
  • Manual), Springer Verlag, 1997. Peter M. Gresshoff, Plant Genome Analysis: Current Topics in Plant Molecular
  • Biology. CRC Press, 1994. John Hammond, Peter McGarvey, Vidadi usibov, Plant Biotechnology.

 

 

Academic Year: 
2019-2020
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