Coming soon
MOLECULAR BIOLOGY AND CYTOGENETICS-P
This course will enable the students to –
- To demonstrate technical knowledge and develop skill set that creates different types of professionals in the field of Botany like in research and development, teaching government and public services.
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Course |
Learning Outcome (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Course Code |
Course title |
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25DBOT 704
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MOLECULAR BIOLOGY AND CYTOGENETICS-P
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CO77: Develop holistic competencies vis-a-vis verbal and written communication skills, which will promote curiosity, innovation, and a passion for research including critical thinking CO78: Understand and evaluate the various technique of DNA isolation, SDS-PAGE and gel electrophoresis. CO79: Analyse and develop knowledge of Stage micrometer and Ocular micrometer. Understand and investigate the induction of polyploidy, study of pollen viability and cytogenetic techniques. CO80: Contribute effectively in course-specific interaction. |
Approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching Learning activities for the student: Self-learning assignment, Effective questions, Stimulation, Seminar presentation, Substantial laboratory-based practical component and experiments Technology-enabled learning
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Practical assignments and laboratory reports Observation of practical skills Individual and group project reports
Viva voce interviews are majorly adopted assessment methods |
CONTENTS:
- Isolation of RNA from Yeast
- Restriction Digestion
- Agarose – Gel Electrophoresis
- SDS- PAGE
- Isolation of DNA from plant material
- Study of general cytological techniques
- Study of pollen viability
- To measure different types of plant cells using Stage micrometer and Ocular micrometer.
- Induction of polyploidy in plants by treating shoot apical meristem with colchicine solution.
- Demonstration of Cytogenetical techniques like physical mapping, RAPD and RFLP
Scheme of Evaluation
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Continuous Assessment |
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Semester End Examination |
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Component |
Marks |
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Component |
Marks |
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Test |
10 |
Isolation of DNA, RNA, Gel Electrophoresis, SDS- PAGE |
20 |
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Attendance |
05 |
Plant cell measurement by Stage micrometer and Ocular micrometer, study of polyploidy, pollen viability RAPD & RFLP. |
20 |
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Spotting |
10 |
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Viva voce |
05
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Viva voce |
10 |
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Practical Record |
10 |
Practical Record |
10 |
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Total |
30 |
Total |
70 |
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Journals:
- Plant Biotechnology Journal (Wiley Online Library)
- Plant Biotechnology Reports (Springer)
- Journal of Plant Biochemistry and Biotechnology (Springer)
- Plant Biotechnology (Hindwai)
- Plant Biotechnology (Frontiers in Plant Science)
e - Resources:
- Virtual Labs: Web-enabled experiments designed for remote operation
- https://vlab.amrita.edu/
