CELL AND MOLECULAR BIOLOGY

Paper Code: 
BOT 121
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

To provide knowledge about the intricacies of life processes at the molecular and cellular level.

12.00
Unit I: 
Fundamentals of Cell

Structural organization of cell, difference between plant and animal cell; prokaryote and eukaryote cell, types of bonds and specialized plant cell types.

Cell wall: Basic architecture of cell wall and biogenesis.

Plasma membrane: Structure, models, functions; ion carriers, channels and pumps.

                                                                                                                        

12.00

Chloroplast: Structure, biogenesis, genome organization and nucleochloroplastic interactions

Mitochondria: Structure, genome organization and biogenesis.

Plasmodesmata: Structure, functions, comparision with gap junctions.

Plant vacuole: Tonoplast membrane, vacuoles as multifunctional compartments, vacuoles as storage organelle.

Ribosomes: Structure, functions and biosynthesis.         

12.00

Nucleus: Structure, nuclear pore complex, nucleolus, packaging of DNA (nucleosome organization, solenoid model).

DNA: Structure, types (A, B and Z forms), replication, damage and repair mechanisms. RNA: Structure and types of RNA, transcription, RNA splicing, RNA editing.

 

12.00

Other cellular organelles: Structure and functions of Golgi apparatus, lysosomes, endoplasmic reticulum, microbodies

The cytoskeleton: organization of microtubules, microfilaments and intermediate filaments, cytoskeletal accessory proteins.

Protein sorting: Targeting of proteins into endoplasmic reticulum and chloroplast.

 

12.00

Cell cycle:  Regulation of cell cycle, role of cyclins and cyclin dependent kinases.

Apoptosis and Programmed Cell Death: Cytokinesis and cell plate formation and Programmed Cell Death.

                                                                                                                                                

Essential Readings: 

Suggested Books:

 

  • Alberts, B; Bray, D; Lewis, J; Raff, M; Roberts,K and Watson, JD. 1999. Molecular Biology of the Cell. Garland Publishing Inc., New York.
  • Buchanan, BB; Gruissem, W and Jones, RL. 2000. Biochemistry and Molecular Biology of Plants. American Society of Plant Physiologists. Maryland, USA.
  • De Robertis. Cell and Molecular Biology. Waverly International.
  • De, DN. 2000. Plant Cell Vacuoles: An Introduction. CSIRO Publication. Collingwood, Australia.
  • Karp and Gerald. Cell and Molecular Biology: Concepts and Experiments (3rd edition). John Wiley & Sons.
  • Kleinsmith, LJ and Kish, VM. 1995. Principles of Cell and Molecular Biology (2nd edition). Harper Collins College Publishers. New York, USA.
  • Krishnamurthy, KV. 2000. Methods in Cell Wall Cytochemistry. CRC Press,Boca Raton; Florida.
  • Lewin, B 2000. Genes VII. Oxford University Press, New York.
  • Lodish, H; Berk, A; Zipursky, SL; Matsudiara, P; Baltimore, D and Darnell, J. 2000. Molecular Cell Biology (4th edition). WH Freeman & Co., New York.
  • Nath, P. Molecular Insight in Plant Biology.
  • Rost, T et al. 1998. Plant Biology. Wordsworth Publishing Co. California, USA. Wolfe, SL. 1993. Molecular and Cellular Biology. Wordsworth Publishing Co. California, USA.
  • Weil, JH. 2003. Molecular Insight in Plant Biology. Oxford & IBH Publishing Co. Pvt. Ltd.

 

 

 

References: 

·         Khasim, SM. 2002. Botanical Microtechnique: Principles and Practice. Capital   

·         Publishing Co., New Delhi.

·         Protein Expression: A Practical Approach. Ed. Higgins, SJ and Hames, BD.

·         Oxford University Press

·         Malacinski, GM and Freifelder, D. Essentials of Molecular Biology III Ed.

Jones & Barlett Publishers

Academic Year: 
2015-2016
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