Coming soon
Botany Lab I
This course will enable the students to –
- To demonstrate technical knowledge and develop skill set that creates different types of professionals in the field of Botany like in research and development, teaching government and public services.
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Course |
Course Outcomes |
Learning and teaching strategies |
Assessment Strategies |
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Course Code |
Course Title |
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25BOT125
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Botany Lab I
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CO25: Develop holistic competencies viv-a-vis verbal and written communication skills, which will promote curiosity, innovation, and a passion for research including critical thinking CO26: Develop skills and design experiments to study cytology and molecular biology techniques. CO27: Improve and maximize knowledge of Cyanobacteria, Algae & Bryophytes and their role in sustainability. Evaluate & standardise protocols for experiments in microbiology, mycology & plant pathology & also propose solutions for disease management. CO28: Contribute effectively in course-specific interaction. |
Approach in Teaching: Substantial laboratory-based practical components and experiments Learning Activities for the students: Peer teaching, Model preparation, Field-based learning Project-based learning |
Practical assignments and laboratory reports Observation of practical skills Individual and group project reports
Viva voce iinteractions are majorly adopted assessment methods |
- Isolation of RNA from Yeast
- Restriction Digestion
- Agarose – Gel Electrophoresis
- SDS- PAGE
- Isolation of DNA from plant material
- Study of general cytological techniques
- Study of various stages of mitosis in Onion root tips
- Study of various stages of meiosis in Onion flower bud
- Calculation of mitotic index
- To study B chromosomes in plants
- Study of pollen viability
- To measure different types of plant cells using Stage micrometer and Ocular micrometer
- Induction of polyploidy in plants by treating shoot apical meristem with colchicine solution
- Preparation of report on Indian contributors in the field of Phycology and Bryology
- Microscopic examination of stained cell preparation of Cyanobacteria by various
- Staining methods
- Microscopic study of representative members of Algae and Bryophytes.
- Estimation of protein content in Spirulina, the Single Cell Protein
- Study of different types of lichen thalli and their role in succession
- List some Palaeobotanical evidences of members belonging to Algae and Bryophytes
- Cleaning of glassware
- To study different methods of sterilization and disinfection by physical agents.
- Instrumentation
- To study various types of culture media
- To determine the growth curve in bacteria (E.coli)
- To study the bacteria by Gram staining
- To become acquainted with Kirby-Baur procedure
- Wine production by fermentative activities of Yeast cells
- Isolation of microorganisms from the mixed culture by spread plate methods
- Isolation of microorganism by pour plate method
- To study ecological relationship between microorganisms of soil by Wino Gradsky Column
- Survey of dermatological diseases caused by Fungi
- Inventory preparations of antibiotics procured from Fungi
- Morphological study of representative members of Fungi- Pilobolus, Chaetomium, Morchella, Melampsora, Polyporus, Dreschlera, Phoma, Peziza, Puccinia
- Symptomatology, identification, causal organism of the following diseases: White rust, bunt, smut, green ear, smut and ergot of Bajra, rust and smut of wheat, paddy blast, cotton wilt, downy mildew and powdery mildew of grapes, tundu, tobacco mosaic virus, little leaf of brinjal, root knot of vegetables
- Report on eminent contributors in Mycology, Plant pathology and important related institutes.
M.Sc. Semester I
25B0T125
Scheme of Evaluation
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Continuous Assessment |
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Semester End Examination |
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Component |
Marks |
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Component |
Marks |
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Test |
10 |
Exercise based on Cell & Molecular Biology |
10 |
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Attendance |
05 |
Exercise based on Cytology & Genetics |
10 |
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Lab Interaction/Viva voce |
05 |
Exercise based on Hierarchy of Plant Kingdom |
10 |
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Exercise based on Fungi, Plant Pathology & Microbiology |
10 |
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Practical Record |
10 |
Spotting |
10 |
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Practical Record |
10 |
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Total |
30 |
Viva voce |
10 |
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Total |
70 |
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Books Recommended:
- Santra, SC and Chatterjee. 2006. College Botany Practical Vol. I. New Central Book Agency (P) Ltd., Calcutta.
- Cavers, F. (2008). Practical botany. Whitefish, MT: Kessinger Pub.
- Bendre, A. (2015). Practical Botany. S.L.: Rastogi Publications.
- Santra, S.C. (2010). College Botany Practical Vol. II. New Central Book Agency (P) Ltd.
e-Resources:
- https://microbiologyinfo.com/gram-staining-principle-procedure-interpretation-examples-and-animation/
- https://fire.biol.wwu.edu/cmoyer/zztemp_fire/biol346_W06/labman_week4.pdf
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Microbiology_Labs_II/Lab_03%3A_Obtaining_Pure_Cultures_from_a_Mixed_Population
- https://www.youtube.com/watch?v=nViO9Y4Yxfk
- https://www.youtube.com/watch?v=KFZYeApJ1Ls
- https://www.youtube.com/watch?v=nk37v3AXlbc
- https://www.youtube.com/watch?v=BhXoT0TGNsI
- https://www.youtube.com/watch?v=bm99zrq3ijo
- https://bio.libretexts.org/Courses/North_Carolina_State_University/MB352_General_Microbiology_Laboratory_2021_(Lee)/04%3A_Staining_Techniques/4.03%3A_Lab_Procedures-_Bacterial_Smear_Simple_and_Gram_Staining
- https://www.youtube.com/watch?v=N-nIHhncEao
- https://www.youtube.com/watch?v=9HwPCb2QdCk
