Coming soon
ADVANCED PLANT PHYSIOLOGY-P
- To demonstrate practices and procedures that lead to acquisition of skills required for creating different types of professionals in the field of Botany like in research and development, teaching government and public services.
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Course |
Learning Outcome (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Course Code |
Course title |
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25DBOT806
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ADVANCED PLANT PHYSIOLOGY-P
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CO122: Develop holistic competencies vis a vis verbal & written communication skill which will promote curiosity, innovation and a passion for research including critical thinking. CO123: Apply practical skills of plant physiology for development of employment/ entrepreneurial ability. CO124: Investigate fundamental concepts in plant physiology, including plasmolysis, osmosis, imbibition, root pressure, transpiration, photosynthesis, respiration, plant growth, phytochemistry, signaling, and stress responses. Emphasis will be placed on developing practical skills in experimental design, data collection, analysis, and interpretation, as well as critical thinking. CO125: Contribute effectively in course-specific interaction. |
Approach in teaching: Substantial laboratory-based practical component, Reading assignments, Demonstration, Team teaching Learning activities for the student: Self-learning assignment, Effective questions, Stimulation, Seminar presentation, Field-based learning Project-based learning |
Practical assignments and laboratory reports Observation of practical skills Individual and group project reports
Viva voce interviews are majorly adopted assessment methods
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CONTENTS:
- Demonstration of phenomenon of osmosis by using potato osmometer.
- Demonstration of phenomenon of imbibition.
- Demonstration of the stomatal transpiration by four leaves method.
- To demonstrate that oxygen is evolved during photosynthesis by inverted funnel method.
- To demonstrate that CO2, water, light and chlorophyll are essential for photosynthesis by ‘Moll’s half leaf’ experiment.
- To determine the chlorophyll a / chlorophyll b ratio in C3 and C4 plants.
- Isolation of intact chloroplasts and estimation of chloroplast proteins by spot protein assay.
- Extraction of chloroplast pigments from leaves and preparation of the absorption spectrum of chlorophylls and carotenoids.
- To demonstrate photophosphorylation in intact chloroplasts, resolve the phosphoproteins by SDS-PAGE and perform autoradiography.
- To determine the value of RQ of different respiratory substrates using Ganong’s respirometer.
- To estimate enzyme activity in plant tissues.
- To measure of growth using auxanometer.
- To study the effect of plant growth regulators on plant growth.
- To separate the given mixture of dyes using paper chromatography.
- To separate the given mixture of amino acids using thin - layer chromatography.
- To separate the given mixture of pigments using thin – layer chromatography.
- To study principles of colorimetry and spectrophotometry.
- Tests for – Alkaloids, Terpenes, Tannins and Anthocyanins
Scheme of Evaluation
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Continuous Assessment |
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Semester End Examination |
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Component |
Marks |
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Component |
Marks |
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Test |
10 |
Exercises on Plant Physiology I |
20 |
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Attendance |
05 |
Exercises on Plant Physiology II |
20 |
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Spotting |
10 |
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Viva voce |
05
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Viva voce |
10 |
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Practical Record |
10 |
Practical Record |
10 |
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Total |
30 |
Total |
70 |
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- Ramakrishnan, S., Prasannan, K. G., & Rajan, R. (2001). Textbook of medical biochemistry. Hyderabad: Orient Longman.
- Plummer, D. T. (2001). Introduction to Practical Biochemistry. India: Tata McGraw Hill Publishing Company.
- Singh, S.P. (2007) Practical Manual of Biochemistry. CBS Publisher, New Delhi.
- Bajracharya, D. (2003). Experiments in plant physiology: A laboratory manual. New Delhi: Narosa.
- Santra, S.C. (2015). College Botany Practical Vol.II. New Central Book Agency (P) Ltd., Calcutta.
- Bendre, A.M. (2009). A Text Book of Practical Botany. Rastogi Publications, Meerut.
- Pandey,S.N., Misra, S.P., Trivedi, P.C. (2016). Botany Vol. II. Vikas Publishing House.
- Pandey,S.N., Misra, S.P., Trivedi, P.C. (2018). Botany Vol. III. Vikas Publishing House
e-Resources:
- shttps://onlinecourses.nptel.ac.in/noc22_bt22/preview Biochemistry IITM
- https://onlinecourses.swayam2.ac.in/cec20_bt12/preview Biochemistry of Biomolecules
- https://onlinecourses.swayam2.ac.in/cec21_ge31/preview Biodiversity of Ecological Resources
- https://onlinecourses.swayam2.ac.in/cec21_bt11/preview Biomolecules: Structure Function in Health and Disease
- https://onlinecourses.nptel.ac.in/noc22_ge12/preview Environmental Chemistry and Microbiology
