Advanced Plant Biotechnology I

Paper Code: 
BOT 421 A
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course Objectives:

This course will enable the students to –

  1. To develop an understanding of latest trends and various tools and techniques of Plant Biotechnology for human welfare.
  2. To impart knowledge about the techniques of plant tissue culture
  3. To study haploid production
  4. To have a basic understanding of the plant genetic transformation
  5. To gain knowledge on Cryopreservation
  6. To know the application of plant tissue culture

 

Course Outcomes (COs):

     Course

Course Outcome

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BOT 421A

 

Elective 1A: Advanced Plant Biotechnology I

On completion of this course, the students will be able to:

CO88:  To focus and recall the core concepts and fundamentals of plant biotechnology, bioreactors and genetic engineering

CO89:  To Demonstrate and explain the competency of different types of plant tissue culture

CO90:  Analyse and interpret the role of different enzymes and vectors used for genetic manipulations

CO91: To Explain gene Cloning and summarize and implement different methods for successful gene transfer in living systems.

CO92: To attribute the major concerns and applications of transgenic technology

CO93:  To interpret and explain the basic concepts of creating a functional Green House and its benefits in the current scenario.

CO94:  To summarize and interpret the significance of secondary metabolites in tissue culture and their commercialization

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question preparation

 

 

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects
 

 

12.00
Unit I: 
Plant Tissue Culture

 

 

Plant Tissue Culture: PTC lab, media preparation and handling, cell and plant culture, sterile techniques, totipotency, various plant tissue culture media and their composition. Micropropagation: organogenesis, somatic embryogenesis, axillary bud proliferation.

12.00
Unit II: 
Recombinant DNA Technology

Recombinant DNA Technology: Cutting and joining DNA molecules, cloning vectors: plasmids, cosmids, bacteriophage vectors, phagemids, YAC, shuttle vectors, expression vectors, gene cloning, direct DNA transfer: particle bombardment, electroporation, liposome mediated transformation, macroinjection; Agrobacterium mediated transformation of plants; in planta gene transfer.

 

12.00
Unit III: 
Organ Culture

Organ Culture:  Anther culture, mature and immature zygotic embryo culture, ovary culture, somaclonal and gametoclonal variations, triploid production, production of virus free plants. Tissue culture in germplasm conservation: Synthetic seeds, Cryopreservation.                                                                                                                                     

 

12.00
Unit IV: 
Greenhouse Hardening Unit:

Greenhouse Hardening Unit:  Greenhouse Construction, types of greenhouses, environmental control inside a greenhouse. Soilless media for propagating and growing nursery plants. Media components: Sand, peat moss, coco-peat, vermiculite, pumice, perlite synthetic plastic aggregate and composts. Preplanting treatments of soil and soil mixes: heat treatments, fumigation and chemicals. 

 

12.00
Unit V: 
Secondary metabolite production in culture

Secondary metabolite production in culture: Biotransformation, factors affecting production of secondary metabolites, production of important drugs in culture- taxol, ginseng, morphine, codeine, vinblastine and vincristine, types of bioreactors (stirred tank, air lift, membrane type, immobilized cell bioreactors).

 

 

Essential Readings: 

Essential Readings

  • Bhojwani, S.S. (2012). Plant Tissue Culture: Applications and Limitations.  Netherlands: Elsevier Science.
  • Razdan, M. K. (2003). Introduction to Plant Tissue Culture. United Kingdom: Science Publishers.
  • Bhojwani, S., Razdan, M. (1996). Plant Tissue Culture: Theory and Practice. Netherlands: Elsevier Science.
  • Soh, W. Y., and Bhojwani, S.S. (2013). Morphogenesis in Plant Tissue Cultures.  Germany: Springer Netherlands.
  • Dantu, P. K., Bhojwani, S. S. (2013). Plant Tissue Culture: An Introductory Text. India: Springer India.
  • Chawla, H. S. (2011). Introduction to Plant Biotechnology (3/e). Jersey: CRC Press.
  • Scott, N., Slater, A., Fowler, M. (2008). Plant biotechnology: the genetic manipulation of plants. United Kingdom: OUP Oxford.

 

Suggested Readings:

  • Benson, E.E. (2002). Plant Conservation Biotechnology.  United Kingdom: Taylor & Francis.
  • Agnès Ricroch, ‎Surinder Chopra, ‎Shelby J. Fleischer · Plant Biotechnology: Experience and Future Prospects. (2014). Germany: Springer International Publishing.
  • Brown, T. A. (2018). Genomes 4. United States: CRC Press.
  • Collin, H., Edwards, S. (1998). Plant Cell Culture. Singapore: BIOS Scientific Publishers.
  • Wilson, C. M., Meredith, C. P., Hollaender, A., Kosuge, T. (2012). Genetic Engineering of Plants: An Agricultural Perspective. United States: Springer US.
  • Gyana Ranjan Rout, ‎K.V. Peter · 2018 (Ed.). Genetic Engineering of Horticultural Crops. (2018). United Kingdom: Elsevier Science.
  • Roberts, L., Staff, N. R. C. (1984). Genetic Engineering of Plants: Agricultural Research Opportunities and Policy Concerns. United States: National Academies Press.
  • Arencibia, A. (2000). Plant Genetic Engineering: Towards the Third Millennium. Netherlands: Elsevier Science.
  • John H. Dodds, ‎John Hamilton Dodds ·Plant Genetic Engineering. (1985). India: Cambridge University Press.

 

References: 

 

e-Resources:

 

Journals:

  • Plant Cell, Tissue and Organ Culture (Springer)
  • In Vitro Cellular and Developmental Biology- Plant (Springer)
  • Plant Biotechnology Journal (Wiley Online Library)
  • Plant Biotechnology Reports (Springer)

 

Academic Year: 
2023-2024
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