Coming soon
Advanced Plant Biotechnology I
Course Objectives:
This course will enable the students to –
- To develop an understanding of latest trends and various tools and techniques of Plant Biotechnology for human welfare.
- To impart knowledge about the techniques of plant tissue culture
- To study haploid production
- To have a basic understanding of the plant genetic transformation
- To gain knowledge on Cryopreservation
- To know the application of plant tissue culture
Course Outcomes (COs):
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Course |
Course Outcome |
Learning and teaching strategies |
Assessment Strategies |
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Paper Code |
Paper Title |
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BOT 421A
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Elective 1A: Advanced Plant Biotechnology I |
CO88: Understand the core concepts and fundamentals of plant biotechnology, bioreactors and genetic engineering CO89: Develop their competency on different types of plant tissue culture CO90: Analyze the enzymes and vectors for genetic manipulations CO91: Examine gene Cloning and evaluate different methods of gene transfer CO92: Critically analyze the major concerns and applications of transgenic technology CO93: Understand the basic concepts of structure of Green House and its function CO94: Know about the significance of secondary metabolites in tissue culture |
Class lectures Seminars Tutorials Group discussions and Workshops Question preparation
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Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects |
PTC lab, media preparation and handling, cell and plant culture, sterile techniques, totipotency, various plant tissue culture media and their composition. Micropropagation: organogenesis, somatic embryogenesis, axillary bud proliferation.
Cutting and joining DNA molecules, cloning vectors: plasmids, cosmids, bacteriophage vectors, phagemids, YAC, shuttle vectors, expression vectors, gene cloning, direct DNA transfer: particle bombardment, electroporation, liposome mediated transformation, macroinjection; Agrobacterium mediated transformation of plants; in planta gene transfer.
Anther culture, mature and immature zygotic embryo culture, ovary culture, somaclonal and gametoclonal variations, triploid production, production of virus free plants. Tissue culture in germplasm conservation: Synthetic seeds, Cryopreservation.
Greenhouse Construction, types of greenhouses, environmental control inside a greenhouse. Soilless media for propagating and growing nursery plants. Media components: Sand, peat moss, coco-peat, vermiculite, pumice, perlite synthetic plastic aggregate and composts. Preplanting treatments of soil and soil mixes: heat treatments, fumigation and chemicals.
Biotransformation, factors affecting production of secondary metabolites, production of important drugs in culture- taxol, ginseng, morphine, codeine, vinblastine and vincristine, types of bioreactors (stirred tank, air lift, membrane type, immobilized cell bioreactors).
