Advanced Plant Biotechnology I

Paper Code: 
BOT421A
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course Objectives:

This course will enable the students to –

  1. To develop an understanding of latest trends and various tools and techniques of Plant Biotechnology for human welfare.
  2. To impart knowledge about the techniques of plant tissue culture
  3.  To study haploid production
  4.  To have a basic understanding of the plant genetic transformation
  5.  To gain knowledge on Cryopreservation
  6.  To know the application of plant tissue culture

 

Course Outcomes (COs):

     Course

Course Outcome

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BOT 421A

 

Elective 1A: Advanced Plant Biotechnology I

CO88:  Understand the core concepts and fundamentals of plant biotechnology, bioreactors and genetic engineering

CO89:  Develop their competency on different types of plant tissue culture

CO90:  Analyze the enzymes and vectors for genetic manipulations

CO91:  Examine gene Cloning and evaluate different methods of gene transfer

CO92:  Critically analyze the major concerns and applications of transgenic technology

CO93:  Understand the basic concepts of structure of Green House and its function

CO94:  Know about the significance of secondary metabolites in tissue culture

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question preparation

 

 

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects
 

 

12.00
Unit I: 
Plant Tissue Culture

PTC lab, media preparation and handling, cell and plant culture, sterile techniques, totipotency, various plant tissue culture media and their composition. Micropropagation: organogenesis, somatic embryogenesis, axillary bud proliferation.                                                                                                                                              

12.00
Unit II: 
Recombinant DNA Technology

Cutting and joining DNA molecules, cloning vectors: plasmids, cosmids, bacteriophage vectors, phagemids, YAC, shuttle vectors, expression vectors, gene cloning, direct DNA transfer: particle bombardment, electroporation, liposome mediated transformation, macroinjection; Agrobacterium mediated transformation of plants; in planta gene transfer.

 

12.00
Unit III: 
Organ Culture

Anther culture, mature and immature zygotic embryo culture, ovary culture, somaclonal and gametoclonal variations, triploid production, production of virus free plants. Tissue culture in germplasm conservation: Synthetic seeds, Cryopreservation.                                                                                                                                      

12.00
Unit IV: 
Greenhouse Hardening Unit

Greenhouse Construction, types of greenhouses, environmental control inside a greenhouse. Soilless media for propagating and growing nursery plants. Media components: Sand, peat moss, coco-peat, vermiculite, pumice, perlite synthetic plastic aggregate and composts. Preplanting treatments of soil and soil mixes: heat treatments, fumigation and chemicals. 

 

12.00
Unit V: 
Secondary metabolite production in culture

Biotransformation, factors affecting production of secondary metabolites, production of important drugs in culture- taxol, ginseng, morphine, codeine, vinblastine and vincristine, types of bioreactors (stirred tank, air lift, membrane type, immobilized cell bioreactors).

 

Essential Readings: 
  • Altman, A. Agricultural Biotechnology. CRC Press.
  • Chakrabarty, AM; Anderson, WA and Moo-Young. 1996. Environmental Biotechnology – Principles & Applications.
  • Chatterjee, AK. Introduction to Environmental Biotechnology. Prentice- Hall of India Pvt. Ltd., New Delhi
  • Chawla, HS. 2002. Introduction to Plant Biotechnology. Science Publishers
  • Cheremisinoff, NP. 1996. Biotechnology for Waste and Waste water Treatment. CABI Publishing.
  • Davis, AR. Biotechnology: Fundamentals, Applications and Recent Developments. Mangal Deep Publications, Jaipur.
  • Edwards and Sue. 1998. Plant Cell Culture. Bios Scientific Publishers, London.
  • Glazer, AN and Nikaido, H. Fundamentals of Applied Microbiology. Cambridge Univ. Press.
  • Greshoff, P.  Plant Biotechnology & Development.  CRC Press.
  • Gupta, PK. Elements of Plant Biotechnology. Rastogi Publications.,Meerut.
  • Ignacimuthu, S. 2001. Plant Biotechnology. Oxford & IBH Publishing Co. Pvt. Ltd
  • Jane, B. Agricultural Biotechnology. Diane Publishing Co.Kumar, U. 2001. Methods in Plant Tissue Culture. Agrobios, Jodhpur.
  • Martin, FMartin, Fussenegger and Al-Rubeai, M. 2007. Systems Biology. Springer Publications.
  • Old & Primrose. Principles of Gene Manupilations.
  • Pathade, GR. Environmental Pollution & Management of Waste Water by Microbial Techniques.Primrose, SB. 1995.
  • Principles of Genome Analysis. Blackwell ScienceLtd. Oxford, UK.
  • Scheper, T. Metabolic Engineering. Springer Publications.
  • Thakur and Shekar, I. Environmental Biotechnology: Basic concepts and Applications.
  • Maina Keru.Ethical Biotechnology. Global Vision Publishing House, Delhi

 

 

Laboratory Readings

 

Academic Year: 
2019-2020
  • Printer-friendly version
  • PDF version