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Practical [1]

Paper Code: 
BOT 503
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course Objectives: 

This course will enable the students to-

  1. To develop skills related to qualitative and quantitative estimations of biomolecules
  2. To observe and analyse various physiological process and pathways.

Course Outcomes (COs):

Course

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BOT 503

 

Practical

At the end of the course the students will be able to:

CO78: Basic understanding of the physiological mechanisms of plants.

CO79:  Acquire a general knowledge of the physical, chemical properties and metabolism of carbohydrates and lipids in living system.

CO80: Apply basic knowledge of biomolecules such as carbohydrates, lipids, protein, nucleic acid and enzymes and physiological functions for entrepreneurial development.

CO81:  Plan the experiments efficiently keeping in view the fundamental biochemical principles of enzymes in living system.

 

Substantial laboratory-based practical component and experiments

 

Technology-enabled learning

 

Field-based learning

Project-based learning

 

Practical assignments and laboratory reports

Observation of practical skills

Individual and group project reports

Viva voce interviews are majorly adopted assessment methods

 

 
 

 

 

 

 

 

 

 

  • Demonstration of phemomenon of plasmolysis using Rheo discolor leaves.
  • Demonstration of phemomenon of osmosis by potato osmoscope.
  • Demonstration of phemomenon of imbibition.
  • Demonstration of phemomenon of root pressure.
  • Demonstration of phemomenon of unequal transpiration in dorsiventral leaves using cobalt chloride paper.
  • Measurement of the rate of transpiration using potometers.
  • Anatomical study of root nodule depicting symbiotic relationship between nitrogen fixing bacteria and roots of leguminuous plants.
  • To demonstrate that light, CO2 and chlorophyll are necessary for photosynthesis.
  • To demonstrate that O2 is evolved during photosynthesis.
  • To determine the value of RQ of different respiratory substrates by Ganong’s respiroscope.
  • To demonstrate that CO2 is given out during respiration
  • Measurement of growth by arc auxanometer.
  • Phytochemical tests for the following- glucose, starch, proteins, fats, tannins, ascorbic acid and anthocyanins.
  • Demonstration of respiratory enzymes- peroxidase, catalase and dehydrogenase in plant tissue samples and measurement of their activities.Ramakrishnan, S; Prasannan, KG and Rajan, R. Textbook of Medical Biochemistry (2nd edition). Orient Longman.
  • Methods in Plant Biochemistry and Molecular Biology. 1997. Dashek, WV (Ed.). CRC Press.
  • Wilson and Walker. Practical Biochemistry: Principles and Techniques. Cambridge University Press. U.K.

 

 

 

 

Essential Readings: 
  • Dashek, W. V. (2018). Methods in plant biochemistry and molecular biology. CRC Press.
  • Wilson, K., Wilson, K., & Walker, J. (2005). Practical Biochemistry: Principles and Techniques. U.K: Press Syndicate of the University of Cambridge.
  • Bala, M., Gupta, S., Gupta N.K. (2013). Practicals in Plant Physiology and Biochemistry.  Scientific Publishers.
  • Rayner, M. C., Keeble, F. (1911). Practical Plant Physiology. United Kingdom: G. Bell and sons, Limited.
  • Acton, E. H., Darwin, F. (2011). Practical Physiology of Plants. United Kingdom: Cambridge University Press.
  • Choudhuri, M. A., & Gupta, K. K. (2020). Practical Plant Physiology. La Vergne: New Central Book Agency.
  • Bendre, A. (2020). Practical botany: Plant physiology, biochemistry, biotechnology, ecology and plant utilization. Meerut, India: Rastogi Publications.
  • Santra, S. C. (2020). Practical Botany. La Vergne: New Central Book Agency.
  • Harborne, J. B. (2012). Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis, 3E. India: Springer.
  • Damodaran, G. K., K, G. D. (2016). Practical Biochemistry. India: Jaypee Brothers Medical Publishers Pvt. Limited.
  • Soundravally, A., Dhiman, P., R, (2019). Biochemistry Practical Manual E-Book. books.google.co.in › books
  • Wood, E. J. (2012). Practical Biochemistry for Colleges. United Kingdom: Elsevier Science.
  •  Sawhney, S. K. (2005). Introductory Practical Biochemistry. Unitedju Kingdom: Alpha Science International.

 

 

 

 

 

 

 

 

References: 

e-Resourses:

 

Academic Year: 
2022-2023 [8]

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Source URL: https://botany.iisuniv.ac.in/courses/subjects/practical-53

Links:
[1] https://botany.iisuniv.ac.in/courses/subjects/practical-53
[2] https://vlab.amrita.edu/
[3] https://bsapubs.onlinelibrary.wiley.com/
[4] https://www.researchgate.net/publication/338187599_PLANT_WATER_RELATION
[5] http://kea.kar.nic.in/vikasana/biology_2013/bio_c9.pdf
[6] https://www.researchgate.net/publication/200787272_Carbohydrates
[7] https://www.uou.ac.in/lecturenotes/science/MSCCH-17/CHEMISTRY%20LN%204%20CARBOHYDRATES-converted%20(1).pdf
[8] https://botany.iisuniv.ac.in/academic-year/2022-2023