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ADVANCED PLANT PHYSIOLOGY-P [1]

Paper Code: 
25DBOT806
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
  1. To demonstrate practices and procedures that lead to acquisition of skills required for creating different types of professionals in the field of Botany like in research and development, teaching government and public services.

 

Course Outcomes: 

Course

Learning Outcome

(at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course title

25DBOT806

 

 

ADVANCED PLANT PHYSIOLOGY-P

 

CO122: Develop holistic competencies vis a vis verbal & written communication skill which will promote curiosity, innovation and a passion for research including critical thinking.

CO123: Apply practical skills of plant physiology for development of employment/ entrepreneurial ability.

CO124: Investigate fundamental concepts in plant physiology, including plasmolysis, osmosis, imbibition, root pressure, transpiration, photosynthesis, respiration, plant growth, phytochemistry, signaling, and stress responses. Emphasis will be placed on developing practical skills in experimental design, data collection, analysis, and interpretation, as well as critical thinking.

CO125: Contribute effectively in course-specific interaction.

Approach in teaching: Substantial laboratory-based practical component,

Reading assignments, Demonstration, Team teaching

Learning activities for the student:

Self-learning assignment, Effective questions, Stimulation, Seminar presentation, Field-based learning

Project-based learning

Practical assignments and laboratory reports

Observation of practical skills

Individual and group project reports

 

Viva voce interviews are majorly adopted assessment methods

 

 

     CONTENTS:

  • Demonstration of phenomenon of osmosis by using potato osmometer.
  • Demonstration of phenomenon of imbibition.
  • Demonstration of the stomatal transpiration by four leaves method.
  •  To demonstrate that oxygen is evolved during photosynthesis by inverted funnel method.
  • To demonstrate that CO2, water, light and chlorophyll are essential for photosynthesis by ‘Moll’s half leaf’ experiment.
  • To determine the chlorophyll a / chlorophyll b ratio in C3 and C4 plants.
  • Isolation of intact chloroplasts and estimation of chloroplast proteins by spot protein assay.
  • Extraction of chloroplast pigments from leaves and preparation of the absorption spectrum of chlorophylls and carotenoids.
  • To demonstrate photophosphorylation in intact chloroplasts, resolve the phosphoproteins by SDS-PAGE and perform autoradiography.
  • To determine the value of RQ of different respiratory substrates using Ganong’s respirometer.
  • To estimate enzyme activity in plant tissues.
  • To measure of growth using auxanometer.
  • To study the effect of plant growth regulators on plant growth.
  • To separate the given mixture of dyes using paper chromatography.
  • To separate the given mixture of amino acids using thin - layer chromatography.
  • To separate the given mixture of pigments using thin – layer chromatography.
  • To study principles of colorimetry and spectrophotometry.
  • Tests for – Alkaloids, Terpenes, Tannins and Anthocyanins

 

 Scheme of Evaluation

Continuous Assessment

 

Semester End Examination

 

Component

 

Marks

 

 

Component

 

Marks

 

Test

 

10

Exercises on Plant Physiology I

 

20

Attendance

05

Exercises on Plant Physiology II

 

20

 

Spotting

 

10

Viva voce

05

 

Viva voce

10

Practical Record

 

10

Practical Record

 

10

 

Total

 

30

 

Total

 

70
 

 

Essential Readings: 
  • Ramakrishnan, S., Prasannan, K. G., & Rajan, R. (2001). Textbook of medical biochemistry. Hyderabad: Orient Longman.
  • Plummer, D. T. (2001). Introduction to Practical Biochemistry. India: Tata McGraw Hill Publishing Company.
  • Singh, S.P. (2007) Practical Manual of Biochemistry. CBS Publisher, New Delhi.
  • Bajracharya, D. (2003). Experiments in plant physiology: A laboratory manual. New Delhi: Narosa.
  • Santra, S.C. (2015). College Botany Practical Vol.II. New Central Book Agency (P) Ltd., Calcutta.
  • Bendre, A.M. (2009). A Text Book of Practical Botany. Rastogi Publications, Meerut.
  • Pandey,S.N., Misra, S.P., Trivedi, P.C. (2016). Botany Vol. II. Vikas Publishing House.
  • Pandey,S.N., Misra, S.P., Trivedi, P.C. (2018). Botany Vol. III. Vikas Publishing House           
References: 

e-Resources:

Academic Year: 
2025-2026 [7]

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Source URL: https://botany.iisuniv.ac.in/courses/subjects/advanced-plant-physiology-p-0

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[2] https://onlinecourses.nptel.ac.in/noc22_bt22/preview
[3] https://onlinecourses.swayam2.ac.in/cec20_bt12/preview
[4] https://onlinecourses.swayam2.ac.in/cec21_ge31/preview
[5] https://onlinecourses.swayam2.ac.in/cec21_bt11/preview
[6] https://onlinecourses.nptel.ac.in/noc22_ge12/preview
[7] https://botany.iisuniv.ac.in/academic-year/2025-2026