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MOLECULAR BIOLOGY AND CYTOGENETICS-P [1]

Paper Code: 
25DBOT704
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to –

  1. To demonstrate technical knowledge and develop skill set that creates different types of professionals in the field of Botany like in research and development, teaching government and public services. 

 

Course Outcomes: 

Course

Learning Outcome

(at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course title

25DBOT

704

 

 

MOLECULAR BIOLOGY AND CYTOGENETICS-P

 

CO77: Develop holistic competencies vis-a-vis verbal and written communication skills, which will promote curiosity, innovation, and a passion for research including critical thinking

CO78: Understand and evaluate the various technique of DNA isolation, SDS-PAGE and gel electrophoresis.  

CO79:  Analyse and develop knowledge of Stage micrometer and Ocular micrometer. Understand and investigate the induction of polyploidy, study of pollen viability and cytogenetic techniques.

CO80: Contribute effectively in course-specific interaction.

Approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

Learning activities for the student:

Self-learning assignment, Effective questions, Stimulation, Seminar presentation, Substantial laboratory-based practical component and experiments

Technology-enabled learning

 

Practical assignments and laboratory reports

Observation of practical skills

Individual and group project reports

 

Viva voce interviews are majorly adopted assessment methods
 

 

   CONTENTS:

  • Isolation of RNA from Yeast
  • Restriction Digestion
  • Agarose – Gel Electrophoresis
  • SDS- PAGE
  • Isolation of DNA from plant material
  • Study of general cytological techniques
  • Study of pollen viability
  • To measure different types of plant cells using Stage micrometer and Ocular micrometer.
  • Induction of polyploidy in plants by treating shoot apical meristem with    colchicine solution.
  • Demonstration of Cytogenetical techniques like physical mapping, RAPD and RFLP

 

Scheme of Evaluation

Continuous Assessment

 

Semester End Examination

Component

Marks

 

Component

Marks

 

Test

 

10

Isolation of DNA, RNA, Gel Electrophoresis, SDS- PAGE

 

20

Attendance

05

Plant cell measurement by Stage micrometer and Ocular micrometer, study of polyploidy, pollen viability RAPD & RFLP.

 

20

 

Spotting

 

10

Viva voce

05

 

Viva voce

10

Practical Record

 

10

Practical Record

 

10

 

Total

 

30

 

Total

 

70
 

 

 

 

 

References: 

Journals:

  • Plant Biotechnology Journal (Wiley Online Library)
  • Plant Biotechnology Reports (Springer)
  • Journal of Plant Biochemistry and Biotechnology (Springer)
  • Plant Biotechnology (Hindwai)
  • Plant Biotechnology (Frontiers in Plant Science)

 e - Resources:

 

 

Academic Year: 
2025-2026 [3]

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Source URL: https://botany.iisuniv.ac.in/courses/subjects/molecular-biology-and-cytogenetics-p-0

Links:
[1] https://botany.iisuniv.ac.in/courses/subjects/molecular-biology-and-cytogenetics-p-0
[2] https://vlab.amrita.edu/
[3] https://botany.iisuniv.ac.in/academic-year/2025-2026