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PLANT BIOTECHNOLOGY-P [1]

Paper Code: 
25DBOT602A
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to- To develop skills related to plant tissue culture and transgenes is.

 

Course Outcomes: 

Course

Learning Outcome

(at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course title

25DBOT

602A

 

 

PLANT BIOTECHNOLOGY-P

 

 

CO57: Develop holistic competencies vis a vis verbal & written communication skill which will promote curiosity, innovation and a passion for research including critical thinking.

CO58: Apply the knowledge of PTC like media formulation, sterilization & culture techniques to achieve in vitro conservation

CO59: Analyse & integrate advance technology of biotransformation in culture systems to obtain a standardized protocol for scale-up production of commercially important compounds.

CO60: Contribute effectively in course-specific interaction. 

Approach in teaching: Interactive Lectures, Discussion, Demonstration, Team teaching, Substantial laboratory-based practical component and experiments,

Technology-enabled learning

Learning activities for the student:

Self-learning assignment, Effective questions, Stimulation, Seminar presentation

Practical assignments and laboratory reports

Observation of practical skills

Individual and group project reports

Viva voce interviews are majorly adopted assessment methods
 

 

 

  CONTENTS:

  • Study of laboratory requirements and general techniques of biotechnology.
  • Principle and application of the following- Laminar Air Flow, Autoclave and Ultracentrifugation.
  • Preparation of different types of tissue culture medias and solutions of nutrients and growth regulators
  • Demonstration of inoculation technique, asceptic transfer of explant, optimizing the conditions for direct/ indirect differentiation in different explants to study totipotency, cytodifferentiation and organogenesis.
  • Demonstration of the technique of protoplast isolation and somatic embryogenesis in Daucus carota.
  • Demonstration of the technique of anther culture in Datura.
  • Demonstration of the technique of gel electrophoresis of bacterial DNA.
  • Demonstration of the phenomenon of action of Restriction Endonucleases.

                                                   

 

 Scheme of Evaluation

Continuous Assessment

 

Semester End Examination

 

Component

 

Marks

 

 

Component

 

Marks

 

Test

 

10

Study of PTC Lab requirements, preparation of different types of tissue culture medias and inoculation technique.

 

20

Attendance

05

Gel electrophoresis, restriction endonucleases, protoplast isolation culture and anther culture.

 

20

 

Spotting

 

10

Viva voce

05

 

Viva voce

10

Practical Record

 

10

Practical Record

 

10

 

Total

 

30

 

Total

 

70
 

 

 

Essential Readings: 
  • Archana, R. (2020). Practical Plant Biotechnology and Genetics. New Delhi: New India Publishing    Agency (NIPA.)
  • Kung, S., & Arntzen, C. J. (1989). Plant biotechnology. Boston: Butterworths.
  • Purohit, S. S. (2007). Practical plant biotechnology. Jodhpur: Student Edition.
  • Ashok M. BendreAshok Kumar. (2010). A Textbook of Practical Botany. Rastogi Publications.
  • Kurnaz, I. A. (2015). Techniques in Genetic Engineering. United Kingdom: CRC Press.
  • Nagar, S., Adhav. M. (2009). Practical Biotechnology and Plant Tissue Culture: S. Chand Limited.
  • Sherathiya, H. (2013). Practical Manual for Plant Tissue Culture: Basic Techniques of Plant Tissue Culture and Molecular Biology. Germany: GRIN Verlag.
  • Pllaiah, T., Subba Rao, M.V., Sreedevi, E. (2017). Plant Tissue Culture: Theory and Practicals. Scientific Publishers.
  • Reinert, J., Yeoman, M. (2012). Plant Cell and Tissue Culture: A Laboratory Manual. Germany: Springer Berlin Heidelberg.
  • Smith, R.H. (2012). Plant Tissue Culture: Techniques and Experiments. Academic Press.

 

References: 

e-Resources:

 

Academic Year: 
2025-2026 [6]

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Source URL: https://botany.iisuniv.ac.in/courses/subjects/plant-biotechnology-p-0

Links:
[1] https://botany.iisuniv.ac.in/courses/subjects/plant-biotechnology-p-0
[2] http://www2.southeastern.edu/Academics/Faculty/jtemple/486/experiment%202.pdf
[3] https://bio.libretexts.org/Bookshelves/Biotechnology/Lab_Manual%3A_Introduction_to_Biotechnology/01%3A_Techniques/1.12%3A_Restriction_Digest_with_Gel_Electrophorisis
[4] https://archive.nptel.ac.in/noc/courses/noc17/SEM2/noc17-bt21/
[5] http://amrita.olabs.edu.in/
[6] https://botany.iisuniv.ac.in/academic-year/2025-2026