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BOTANY LAB IV [1]

Paper Code: 
25BOT423A
Credits: 
6
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to –

  1. To demonstrate and develop specific technical knowledge in field of Plant tissue culture.

 

Course Outcomes: 

Course

 Course Outcomes

Learning and teaching strategies

Assessment Strategies

Course Code

Course Title

25BOT423A

 

(BOTANY LAB IV)

 

CO122: Develop holistic competencies vis a vis verbal & written communication skills which will promote curiosity, innovation and a passion for research including critical thinking

CO 123: Propose experiments for culture media formulation to achieve morphogenesis in superior germplasm.

CO 124: Design protocols for the isolation of protoplast, plasmid and execute the latest techniques used for genome sequencing to produce novel variants.

CO 125: Contribute effectively in course-specific interaction.

 

Substantial laboratory-based practical component and experiments

Technology-enabled learning

Field-based learning

Project-based learning

Internship in industry, and research establishments.

Literature surveys and evaluations

 

Practical assignments and laboratory reports

Observation of practical skills

Individual and group project reports

 

Viva voce interviews are majorly adopted assessment methods

 

 

 

Unit I: 
CONTENTS:
  • Media preparation, sterilization and inoculation of given material.
  • ​Growth characteristics of E.coli using plating and turbid metric methods.
  • Isolation of plasmid from E.coli by alkaline lysis method and its quantitation spectrophotometrically.
  • Restriction digestion of the plasmid and estimation of the size of various DNA fragments.
  • Cloning of a DNA fragment in a plasmid vector, transformation of the given bacterial population and selection recombinants.

  • Organogenesis and somatic embryogenesis using appropriate explants and preparation of artificial seeds.

  • Isolation of protoplast from various plant tissues and testing their viability.

  • Effect of physical and chemical factors protoplast yield.

  • Demonstration of protoplast fusion employing PEG.

  • Demonstration of DNA sequencing using Sanger, s dideoxy method.

  •  Demonstration of micro techniques for plant cultures.

  • Co-cultivation of the plant material with Agrobacterium and study GUS activity histochemically.

​M.Sc. Semester IV

25B0T423A

Scheme of Evaluation

 

Continuous Assessment

 

 

Semester End Examination

 

Component

 

Marks

 

 

Component

 

Marks

 

Test

 

10

 

Exercise based on Plant Tissue Culture

20

 

Attendance

 

05

 

Lab Interaction/Viva voce

 

05

 

Practical Record

 

10

Exercise based on Advanced Plant Biotechnology

20

 

 

 

 

Total

 

 

 

 

30

 

Spotting

 

10

 

Practical Record

 

10

 

 

 

Viva voce

 

10

 

Total

 

70

 

 

 

Unit II: 
Scheme of Evaluation
Essential Readings: 

Books Recommended:

  • Annarita Leva, ‎Laura Rinaldi. Recent Advances in Plant in Vitro Culture. (2012). Croatia: IntechOpen.
  • Bohorova, N. (1999). Laboratory protocols: CIMMYT Applied genetic engineering laboratory. Mexico: CIMMYT, Applied Biotechnology Center.
  • Dodds, J. H., Dodds, J. H., Roberts, L. W. (1995). Experiments in plant tissue culture. United Kingdom: Cambridge University Press.
  • George, E. F. (1993). Plant Propagation by Tissue Culture. United Kingdom: Exegetics.
  • Pierik, R. (2012). In Vitro Culture of Higher Plants. Netherlands: Springer Netherlands.
  • Oluf Gamborg, ‎Gregory C. Phillips ·Plant Cell, Tissue and Organ Culture: Fundamental Methods. (2013). Germany: Springer Berlin Heidelberg.
  • Shri Mohan Jain, ‎Pramod K. Gupta · Protocol for Somatic Embryogenesis in Woody Plants. (2005). Netherlands: Springer Netherlands.
  • . Hvoslef-Eide & A.K,. Preil ‎W (Ed.)· Liquid Culture Systems for in Vitro Plant Propagation. (2005). Netherlands: Springer Netherlands.

 

References: 

e-Resources:

 

Academic Year: 
2025-2026 [7]

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Source URL: https://botany.iisuniv.ac.in/courses/subjects/botany-lab-iv

Links:
[1] https://botany.iisuniv.ac.in/courses/subjects/botany-lab-iv
[2] http://www2.southeastern.edu/Academics/Faculty/jtemple/486/experiment%202.pdf
[3] https://bio.libretexts.org/Bookshelves/Biotechnology/Lab_Manual%3A_Introduction_to_Biotechnology/01%3A_Techniques/1.12%3A_Restriction_Digest_with_Gel_Electrophorisis
[4] https://www.youtube.com/watch?v=_EYsykjsfiY
[5] https://www.youtube.com/watch?v=zB9Vyh9eiaQ
[6] https://www.sigmaaldrich.com/IN/en/technical-documents/protocol/genomics/sequencing/sanger-sequencing
[7] https://botany.iisuniv.ac.in/academic-year/2025-2026